The use of multiple indices of physiological activity to access viability in chlorine disinfected Escherichia coli O157:H7
The use of multiple indices of physiological activity to access viability in chlorine disinfected Escherichia coli O157:H7
复制标题
DOI：
10.1046/j.1365-2672.1999.00572.x
复制
发表时间：
1999-07-01
影响因子：
2.4
通讯作者：
McFeters, GA
中科院分区：
文献类型：
--
作者：
Lisle, JT;Pyle, BH;McFeters, GA
一套荧光细胞内染料和探针，结合活平板计数，以评估氯消毒对膜电位的影响（罗丹明123; Rh 123和双-（1，3-二丁基巴比妥酸）三次甲基氧杂蒽酚; DiBAC（4）（3）），膜完整性（LIVE/DEAD BacLight试剂盒）、呼吸活性（5-氰基-2，3-二甲苯基四唑氯化物; CTC）和底物反应性（直接活菌计数; DVC）。消毒剂作用5 min后，对生理指标的影响顺序为：活菌平板计数>底物反应性>膜电位>呼吸活性>膜完整性。通过检查多个目标的生理活性的原位评估，如在这项研究中所示，允许更全面地确定在细菌细胞亚致死氯消毒后的损伤的部位和程度。这种评估改变的细菌生理学的方法在感兴趣的检测应激细菌的各种领域中具有应用。
A suite of fluorescent intracellular stains and probes was used, in conjunction with viable plate counts, to assess the effect of chlorine disinfection on membrane potential (rhodamine 123; Rh123 and bis-(1,3-dibutylbarbituric acid) trimethine oxonol; DiBAC(4)(3)), membrane integrity (LIVE/DEAD BacLight kit), respiratory activity (5-cyano-2,3-ditolyl tetrazolium chloride; CTC) and substrate responsiveness (direct viable counts; DVC) in the commensal pathogen Escherichia roll O157:H7. After a 5 min exposure to the disinfectant, physiological indices were affected in the following order: viable plate counts > substrate responsiveness > membrane potential > respiratory activity > membrane integrity. In situ assessment of physiological activity by examining multiple targets, as demonstrated in this study, permits a more comprehensive determination of the site and extent of injury in bacterial cells following sublethal disinfection with chlorine. This approach to assessing altered bacterial physiology has application in various fields where detection of stressed bacteria is of interest.