Identification of quantitative trait loci affecting reproduction in pigs.
Identification of quantitative trait loci affecting reproduction in pigs.
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DOI：
10.2527/2001.793623x
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发表时间：
2001-03
影响因子：
3.3
通讯作者：
J. Cassady;R. Johnson;D. Pomp;G. Rohrer;L. D. Van Vleck;E. Spiegel;K M Gilson
中科院分区：
文献类型：
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作者：
J. Cassady;R. Johnson;D. Pomp;G. Rohrer;L. D. Van Vleck;E. Spiegel;K M Gilson
本研究的目的是确定含有影响猪繁殖的 QTL 的染色体区域。通过将随机选择的对照品系（C）中的低指数猪与为增加排卵率和胚胎存活指数而选择的品系中的高指数猪（I）杂交，开发了三代资源群体。第 10 代的 I 系和 C 系之间的差异是，妊娠 50 天时有 6.7 个卵子和 3.3 个胎儿，出生时有 3.1 个完全成形猪和 1.6 个活猪。收集 F2 雌性的表型数据，分三个重复出生，包括排卵率 (n = 423)、青春期年龄 (n = 295)、窝产仔数 (n = 370) 和乳头数量 (n = 428)。产仔数数据包括完全成形、活产、死产和木乃伊猪的数量。对祖代、F1 和 F2 动物进行了基因分型，检测了分布在所有 18 个常染色体和 X 染色体上的 151 个微卫星标记。 423 只 F2 雌性的基因型数据可用。标记之间的平均间距为 19.3 Kosambi 厘摩。比值对数 (LOD) 分数的计算采用最小二乘法，模型中包含母系组合和重复的固定效应。使用排列方法计算出 5% 和 10% 的全基因组显着性水平阈值。有证据表明（P < 0.05）QTL 影响 SSC9 的排卵率、SSC7 和 SSC8 的青春期年龄、SSC8 和 SSC11 的乳头数量、SSC5 和 SSC13 的死产猪数量以及 SSC11 的完全成形猪数量。有证据表明（P < 0.10）额外的 QTL 影响 SSC7、SSC8 和 SSC12 的青春期年龄、SSC11 的活产数量以及 SSC1、SSC6 和 SSC7 的乳头数量。产仔数遗传性低且受性别限制。因此，通过标记辅助选择可以提高窝产仔数选择的准确性。排卵率和青春期年龄很难测量，因此标记辅助选择可以提供实用且有效的选择方法。
The objective of this research was to identify chromosomal regions harboring QTL affecting reproduction in pigs. A three-generation resource population was developed by crossing low-indexing pigs from a randomly selected control line (C) with high-indexing pigs of a line selected for increased index of ovulation rate and embryonic survival (I). Differences between Lines I and C at Generation 10 were 6.7 ova and 3.3 fetuses at 50 d of gestation and 3.1 fully formed and 1.6 live pigs at birth. Phenotypic data were collected on F2 females, born in three replicates, for ovulation rate (n = 423), age at puberty (n = 295), litter size (n = 370), and number of nipples (n = 428). Litter-size data included number of fully formed, live, stillborn, and mummified pigs. Grandparent, F1, and F2 animals were genotyped for 151 microsatellite markers distributed across all 18 autosomes and the X chromosome. Genotypic data were available on 423 F2 females. Average spacing between markers was 19.3 Kosambi centimorgans. Calculations of logarithms of odds (LOD) scores were by least squares, and fixed effects for sire-dam combination and replicate were included in the models. Genome-wide significance level thresholds of 5% and 10% were calculated using a permutation approach. There was evidence (P < 0.05) for QTL affecting ovulation rate on SSC9, age at puberty on SSC7 and SSC8, number of nipples on SSC8 and SSC11, number of stillborn pigs on SSC5 and SSC13, and number of fully formed pigs on SSC11. There was evidence (P < 0.10) for additional QTL affecting age at puberty on SSC7, SSC8, and SSC12, number born live on SSC11, and number of nipples on SSC1, SSC6, and SSC7. Litter size is lowly heritable and sex-limited. Therefore, accuracy of selection for litter size may be enhanced by marker-assisted selection. Ovulation rate and age at puberty are laborious to measure, and thus marker-assisted selection may provide a practical and efficient method of selection.