A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients.
A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients.
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DOI：
10.1080/22221751.2022.2045874
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发表时间：
2022-12
影响因子：
13.2
通讯作者：
Lu F
中科院分区：
文献类型：
--
作者：
Yu G;Chen R;Zheng S;Liu Y;Zou J;Gu Z;Jiang B;Gao Q;Dai L;Peng J;Wang J;Lu F
血清B型肝炎病毒（HBV）前基因组RNA（pgRNA）是反映共价闭合环状DNA转录活性的替代标志物。然而，目前尚无一种定量检测慢性B型肝炎患者血清HBV RNA的标准方法。本研究建立了HBV前C/C区pgRNA（preC/C region HBV pgRNA）、SF-RNA（splicing variants-free pgRNA）和XR-RNA（X region remained pgRNA）的定量PCR方法。分析了3个回顾性队列：35例初治慢性HBV感染患者（队列A）、52例接受核苷（酸）类似物（NAs）治疗48周的慢性B（CH B）患者（队列B）和8例长期接受NAs治疗的CH B患者（队列C）血清pgRNA剪接变异体和3′端截短的动态变化。采用国家标准HBVRNA检测方法评价HBVRNA检测的准确性和敏感性。我们证实，在未经治疗和NAs治疗的CHB患者中，存在高比例的pgRNA剪接变体和3′端截短，并显著影响血清HBV RNA的定量检测。为提高HBV RNA水平检测的准确性和灵敏度，应将引物和探针设计在HBV基因组5′端，并位于pgRNA主要剪接序列之外，尤其是长期NAs治疗的CHB患者。本研究有助于更好地了解pgRNA剪接变异体和3′端截短的意义，进一步指导临床血清HBV RNA的检测。
Serum hepatitis B virus (HBV) pregenomic RNA (pgRNA) is a surrogate marker for reflecting the transcriptional activity of covalently closed circular DNA. However, there is still no standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients. In this study, quantitative polymerase chain reactions for detecting the preC/C-RNA (preC/C region HBV pgRNA), SF-RNA (splicing variants-free pgRNA) and XR-RNA (X region remained pgRNA) regions were set up. The dynamic changes of serum pgRNA splicing variants and 3′ terminal truncations were analysed in three retrospective cohorts: 35 treatment-naive chronic HBV-infected patients (cohort A), 52 chronic hepatitis B (CHB) patients who received nucleos(t)ide analogs (NAs) therapy for 48 weeks (cohort B) and eight CHB patients who are under long-term NAs treatment (cohort C). The accuracy and sensitivity of HBV RNA detection were assessed by the National Standard of HBV RNA. We confirmed that high proportions of pgRNA splicing variants and 3′ terminal truncations were present and significantly affect the quantitative detection of serum HBV RNA in both treatment-naive and NAs-treated CHB patients. To achieve the higher accuracy and sensitivity on the detection of HBV RNA level, the primers and probes should be designed at the 5′ terminal region of HBV genome and outside the mainly spliced sequence of pgRNA, especially for CHB patients under long-term NAs treatment. This study would help to better understand the significance of the pgRNA splicing variants and 3′ terminal truncations, and further guide the clinical detection of serum HBV RNA.
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影响因子：
28.3
作者：
Patel N;White SJ;Thompson RF;Bingham R;Weiß EU;Maskell DP;Zlotnick A;Dykeman E;Tuma R;Twarock R;Ranson NA;Stockley PG
通讯作者：
Stockley PG
通过监测治疗的慢性乙型肝炎患者特征突变的出现和逆转表明乙型肝炎病毒共价闭合环状 DNA 的快速周转
DOI：
10.1002/hep.31240
发表时间：
2021-01
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Huang Q;Zhou B;Cai D;Zong Y;Wu Y;Liu S;Mercier A;Guo H;Hou J;Colonno R;Sun J
通讯作者：
Sun J
影响因子：
5.4
作者：
Bai L;Zhang X;Kozlowski M;Li W;Wu M;Liu J;Chen L;Zhang J;Huang Y;Yuan Z
通讯作者：
Yuan Z
影响因子：
5.4
作者：
Qi, Zhihua;Li, Gaiyun;Deng, Qiang
通讯作者：
Deng, Qiang
影响因子：
13.5
作者：
Cornberg, Markus;Lok, Anna Suk-Fong;Zoulim, Fabien
通讯作者：
Zoulim, Fabien