A combination of exercise and capsinoid supplementation additively suppresses diet-induced obesity by increasing energy expenditure in mice.
A combination of exercise and capsinoid supplementation additively suppresses diet-induced obesity by increasing energy expenditure in mice.
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DOI：
10.1152/ajpendo.00354.2014
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发表时间：
2015-02
期刊：
影响因子：
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通讯作者：
Kana Ohyama;Y. Nogusa;Katsuya Suzuki;Kosaku Shinoda;S. Kajimura;M. Bannai
中科院分区：
文献类型：
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作者：
Kana Ohyama;Y. Nogusa;Katsuya Suzuki;Kosaku Shinoda;S. Kajimura;M. Bannai
运动可以有效预防肥胖和与肥胖相关的疾病，例如2型糖尿病。辣椒素 (CSN) 是在无刺激性辣椒中发现的辣椒素类似物，可增加全身能量消耗。尽管运动和 CSN 都具有抗肥胖功能，但运动补充 CSN 的有效性尚未得到研究。在这里，我们研究了补充 CSN 是否可以进一步增强小鼠运动的有益效果。小鼠被随机分配到四组：1）高脂肪饮食（HFD，对照组），2）含有 0.3% CSN 的 HFD，3）带有自愿跑轮运动的 HFD（运动），4）含有 0.3% CSN 的 HFD 并带有自愿跑轮运动（运动 + CSN）。摄入8周后，收集血液和组织并进行分析。尽管 CSN 在 HFD 下显着抑制了体重增加，但与单独运动相比，补充 CSN 和运动可进一步减少体重增加和脂肪积累，并增加全身能量消耗。运动与 CSN 补充剂相结合可显着改善代谢特征，包括血浆胆固醇水平。此外，这种组合显着预防饮食引起的肝脏脂肪变性，并减少白色脂肪组织中脂肪细胞的大小。运动和 CSN 显着增加棕色脂肪组织 (BAT) 中的 cAMP 水平和 PKA 活性，表明脂肪分解增加。此外，它们显着激活了骨骼肌中的氧化磷酸化基因程序和脂肪酸氧化。这些结果表明，CSN 有效促进运动的抗肥胖作用，部分是通过激活骨骼肌中的脂肪氧化和 BAT 中的脂肪分解来增加能量消耗。
Exercise effectively prevents the development of obesity and obesity-related diseases such as type 2 diabetes. Capsinoids (CSNs) are capsaicin analogs found in a nonpungent pepper that increase whole body energy expenditure. Although both exercise and CSNs have antiobesity functions, the effectiveness of exercise with CSN supplementation has not yet been investigated. Here, we examined whether the beneficial effects of exercise could be further enhanced by CSN supplementation in mice. Mice were randomly assigned to four groups: 1) high-fat diet (HFD, Control), 2) HFD containing 0.3% CSNs, 3) HFD with voluntary running wheel exercise (Exercise), and 4) HFD containing 0.3% CSNs with voluntary running wheel exercise (Exercise + CSN). After 8 wk of ingestion, blood and tissues were collected and analyzed. Although CSNs significantly suppressed body weight gain under the HFD, CSN supplementation with exercise additively decreased body weight gain and fat accumulation and increased whole body energy expenditure compared with exercise alone. Exercise together with CSN supplementation robustly improved metabolic profiles, including the plasma cholesterol level. Furthermore, this combination significantly prevented diet-induced liver steatosis and decreased the size of adipocyte cells in white adipose tissue. Exercise and CSNs significantly increased cAMP levels and PKA activity in brown adipose tissue (BAT), indicating an increase of lipolysis. Moreover, they significantly activated both the oxidative phosphorylation gene program and fatty acid oxidation in skeletal muscle. These results indicate that CSNs efficiently promote the antiobesity effect of exercise, in part by increasing energy expenditure via the activation of fat oxidation in skeletal muscle and lipolysis in BAT.