Re-analysis of a human hepatitis B virus (HBV) isolate from an East African wild born Pan troglodytes schweinfurthii :: Evidence for interspecies recombination between HBV infecting chimpanzee and human
Re-analysis of a human hepatitis B virus (HBV) isolate from an East African wild born Pan troglodytes schweinfurthii :: Evidence for interspecies recombination between HBV infecting chimpanzee and human
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DOI：
10.1016/j.gene.2004.12.021
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发表时间：
2005-04-11
期刊：
影响因子：
3.5
通讯作者：
Hatzakis, AE
中科院分区：
文献类型：
--
作者：
Magiorkinis, EN;Magiorkinis, GN;Hatzakis, AE
根据目前的估计，全世界有20亿人感染了B型肝炎病毒（HBV），其中3.6亿人患有慢性HBV感染。除人类外，HBV或HBV样病毒也已从不同种类的猿和哺乳动物中分离出来。尽管重组已被描述为在人HBV谱系内的不同基因型之间广泛发生，但从未报道过人与非人灵长类HBV序列之间的重组事件。我们的目的是使用bootscanning和系统发育分析，在所有可用的全长人类和非人类灵长类HBV序列中，对人类和非人类灵长类HBV毒株之间的重组进行详尽的搜索。有趣的是，我们发现从东非野生的Pantroglodytes schweinfurthii分离的HBV序列-FG-是由HBV感染黑猩猩（ChHBV）和人类基因型C组成的重组体。更具体地，在约500 nt的片段中（位置551-1050跨越pol的RT结构域的一半，其与小表面蛋白的编码区的一半重叠），FG与HBV基因型C分组，而在基因组的其余部分中，其与ChHBV序列分组。系统发育分析表明，在后者的区域FG是更密切相关的Pan troglodytes troglodytes亚种，形成一个离群的这个群体。此外，我们的证据表明，重组事件发生后，在人类中的HBV基因型C的初始分散。最后，我们的研究结果指出，虽然感染不同物种的HBV病毒之间发生罕见的重组。(c)2004 Elsevier B. V.保留所有权利。
According to current estimates, hepatitis B virus (HBV) has infected 2 billion people worldwide and among them, 360 million suffer from chronic HBV infection. Except humans, HBV or HBV-like viruses have also been isolated from different species of apes and mammals. Although recombination has been described to occur extensively between different genotypes within the human HBV lineage, no recombination event has ever been reported between human and non-human primate HBV sequences. It was our objective to perform an exhaustive search for recombination between human and non-human primate HBV strains among all available full-length human and non-human primate HBV sequences, using bootscanning and phylogenetic analyses. Intriguingly, we found that an HBV sequence isolated from a wild born Pan troglodytes schweinfurthii in East Africa-FG-is a recombinant consisting of HBV infecting chimpanzee (ChHBV) and human genotype C. More specifically, in a fragment of approximately 500 nt (positions 551-1050 spanning half of the RT domain of pol, which overlaps with half of the coding region of the small surface protein), FG grouped with HBV genotype C, while in the rest of the genome it grouped with ChHBV sequences. Phylogenetic analyses showed that in the latter region FG was more closely related to the Pan troglodytes troglodytes subspecies, forming an outlier to this group. Moreover, we show evidence that the recombination event occurred after the initial dispersion of HBV genotype C in humans. Finally, our findings point out that although rare recombination between HBV viruses infecting different species occurs. (c) 2004 Elsevier B.V. All rights reserved.