Silica-coated magnetic nanoparticles loaded with Cu(II): preparation and application to the purification of bovine serum albumin
Silica-coated magnetic nanoparticles loaded with Cu(II): preparation and application to the purification of bovine serum albumin
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DOI：
10.1007/s00604-012-0918-y
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发表时间：
2013-02
影响因子：
5.7
通讯作者：
Xiujuan Jiang;Lingman Huang;Xiaoming Liu
中科院分区：
文献类型：
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作者：
Xiujuan Jiang;Lingman Huang;Xiaoming Liu
制备了包被硅胶的磁性纳米颗粒（MNPs），然后通过硅烷偶联剂（3-氯丙基）三乙氧基硅烷与三齿配体进行功能化，最后负载Cu（II）离子。采用TEM、SEM、XRD、FTIR和TGA等技术对所得材料进行了表征。它们对牛血清白蛋白具有很强的亲和力，吸附量高达235 mg g−1，吸附平衡快速（30 min）建立。重复吸附（6次）对吸附性能影响不大。研究了吸附牛血清白蛋白的动力学和等温线。制备了与Cu（II）结合的三齿配体BPA功能化的FigCore-Shell磁性纳米颗粒。吸附量大（235 mg g−1），吸附平衡快速建立（30 min）。重复吸附-解吸（6次）对吸附量无显著影响。
Magnetic nanoparticles (MNPs) coated with silica gel were prepared, then functionalized with a tridentate ligand via a silane coupling agent (3-chloropropyl)triethoxysilane, and finally loaded with Cu(II) ions. The resulting materials were characterized by TEM, SEM, XRD, FTIR and TGA techniques. They display strong affinity for BSA with an adsorption capacity as high as 235 mg g−1and with a fast (30 min) establishment of adsorption equilibrium. Repetitive adsorptions (6 times) hardly affect the adsorption capability. The kinetics and isotherm of the adsorption of BSA were also investigated.FigCore-Shell magnetic nanoparticles functionalised with tridentate ligand BPA which binds Cu(II) were prepared. The materials adsorb BSA with high adsorption capacity (235 mg g−1) and fast establishment of adsorption equilibrium (30 min). Repetitive adsorption-desorption (6 times) did not affect significantly the adsorption capacity.