Epstein-barr virus-specific RNA. II. Analysis of polyadenylated viral RNA in restringent, abortive, and prooductive infections
Epstein-barr virus-specific RNA. II. Analysis of polyadenylated viral RNA in restringent, abortive, and prooductive infections
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Epstein-barr 病毒特异性 RNA。
作者：
T. Orellana;E. Kieff
通过感染细胞RNA与纯化病毒DNA的杂交，分析了EB病毒（EBV）特异性RNA在EBV限制性、流产性和生产性感染细胞培养物中的复杂性和丰度。数据表明以下情况。(i)含有生产性感染细胞的培养物含有由至少45%的EBV DNA编码的病毒RNA，并且几乎所有种类的病毒RNA都存在于多腺苷酸化和多核糖体RNA组分中。(ii)限制性感染的Namalwa和Raji培养物仅含有核内抗原EBNA，并且体外生长能力增强，含有分别由至少16%和30%的EBV DNA编码的EBV RNA。Raji和Namalwa细胞的多聚腺苷酸化和多聚核糖体RNA组分富含一类由约5%的EBV DNA编码的EBV RNA。Raji和Namalwa细胞的多聚腺苷酸化和多聚核糖体RNA由相同的EBV DNA序列编码。(iii)在用EBV（HR-1）超感染Raji培养物后，流产感染的细胞含有由至少41%的EBV DNA编码的RNA。超感染Raji细胞的多聚腺苷酸化RNA富含由约20%的EBV HR-1 DNA编码的一类EBV RNA。总和杂交实验表明，超级感染的Raji细胞中的多聚腺苷酸化RNA由与超级感染前Raji细胞中存在的编码RNA相同的DNA序列编码，其中大部分没有被多聚腺苷酸化。相同的EBV RNA序列存在于两个独立衍生的，restringently感染的细胞系的多聚腺苷酸化和多聚核糖体组分中，表明这些RNA可能指定与维持转化状态相关的功能。这类RNA的复杂性足以确定至少5,000个氨基酸的序列。只有一些RNA种类是多聚腺苷酸在restricted和流产感染表明，多聚腺苷酸或任何决定多聚腺苷酸可能在EVB基因组的限制性表达中发挥作用。
The complexity and abundance of Epstein-Barr (EBV)-specific RNA in cell cultures restringently, abortively, and productively infected with EBV has been analyed by hybridization of the infected cell RNA with purified viral DNA. The data indicate the following. (i) Cultures containing productively infected cells contain viral RNA encoded by at least 45% of EBV DNA, and almost all of the species of viral RNA are present in the polyadenylated and polyribosomal RNA fractions. (ii) Restringently infected Namalwa and Raji cultures, which contain only intranuclear antigen, EBNA, and enhanced capacity for growth in vitro, contain EBV RNA encoded by at least 16 and 30% of the EBV DNA, respectively. The polyadenylated and polyribosomal RNA fractions of Raji and Namalwa cells are enriched for a class of EBV RNA encoded by approximately 5% of EBV DNA. The same EBV DNA sequences encode the polyadenylated and polyribosomal RNA of both Raji and Namalwa cells. (iii) After superinfection of Raji cultures with EBV (HR-1), the abortively infected cells contain RNA encoded by at least 41% of EBV DNA. The polyadenylated RNA of superinfected Raji cells is enriched for a class of EBV RNA encoded by approximately 20% of EBV HR-1 DNA. Summation hybridization experiments suggest that the polyadenylated RNA in superinfected Raji cells is encoded by the same DNA sequences as encode RNA present in Raji cells before superinfection, most of which is not polyadenylated. That the same EBV RNA sequences are present in the polyadenylated and polyribosomal fractions of two independently derived, restringently infected cell lines suggests that these RNAs may specify functions related to maintenance of the transformed state. The complexity of this class of RNA is adequate to specify a sequence of a least 5,000 amino acids. That only some RNA species are polyadenylated in restringent and abortive infection suggests that polyadenylation or whatever determines polyadenylation may play a role in the restricted expression of the EVB genome.