Endothelial cell responses in terms of adhesion, proliferation, and morphology to stiffness of polydimethylsiloxane elastomer substrates
Endothelial cell responses in terms of adhesion, proliferation, and morphology to stiffness of polydimethylsiloxane elastomer substrates
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DOI：
10.1002/jbm.a.35186
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发表时间：
2015-07-01
影响因子：
4.9
通讯作者：
Abu Osman, Noor Azuan
中科院分区：
文献类型：
--
作者：
Ataollahi, Forough;Pramanik, Sumit;Abu Osman, Noor Azuan
细胞外环境可以调节细胞行为，因为细胞可以主动感知它们的机械环境。本研究评价了内皮细胞在聚二甲基硅氧烷（PDMS）/氧化铝（Al 2 O3）复合材料和纯PDMS上的粘附、增殖和形态学。基材由纯PDMS及其具有2.5、5、7.5和10重量% Al 2 O3的复合材料在50 ℃的固化温度下制备4小时。然后通过机械、结构和形态学分析对基材进行表征。分别采用刃天青法和1，1-双十八烷基-1，3，3，3，3-四甲基吲哚羰花青高氯酸盐乙酰化低密度脂蛋白（Dil-Ac-LDL）细胞染色法，观察培养的牛主动脉内皮细胞（BAEC）在不同基质材料上的粘附、增殖和形态学变化。复合材料（PDMS/2.5，5，7.5，和10重量%的Al 2 O3）表现出更高的刚度比纯PDMS基板。结果还表明，较硬的基板促进内皮细胞粘附和增殖，并诱导蔓延形态的内皮细胞相比，较低的刚性基板。统计分析表明，时间对细胞增殖的影响依赖于刚度。因此，本研究的结论是，除了不同的Al 2 O3百分比的PDMS基板硬度升高，这反过来又增加了内皮细胞的粘附和增殖显着诱导梭形形态的内皮细胞。(c)2014 Wiley Periodicals，Inc. J Biomed Mater Res Part A：103：2203-2213，2015.
Extracellular environments can regulate cell behavior because cells can actively sense their mechanical environments. This study evaluated the adhesion, proliferation and morphology of endothelial cells on polydimethylsiloxane (PDMS)/alumina (Al2O3) composites and pure PDMS. The substrates were prepared from pure PDMS and its composites with 2.5, 5, 7.5, and 10 wt % Al2O3 at a curing temperature of 50 degrees C for 4 h. The substrates were then characterized by mechanical, structural, and morphological analyses. The cell adhesion, proliferation, and morphology of cultured bovine aortic endothelial (BAEC) cells on substrate materials were evaluated by using resazurin assay and 1,1-dioctadecyl-1,3,3,3,3-tetramethylindocarbocyanine perchlorate-acetylated LDL (Dil-Ac-LDL) cell staining, respectively. The composites (PDMS/2.5, 5, 7.5, and 10 wt % Al2O3) exhibited higher stiffness than the pure PDMS substrate. The results also revealed that stiffer substrates promoted endothelial cell adhesion and proliferation and also induced spread morphology in the endothelial cells compared with lesser stiff substrates. Statistical analysis showed that the effect of time on cell proliferation depended on stiffness. Therefore, this study concludes that the addition of different Al2O3 percentages to PDMS elevated substrate stiffness which in turn increased endothelial cell adhesion and proliferation significantly and induced spindle shape morphology in endothelial cells. (c) 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103: 2203-2213, 2015.