High Performance Liquid Chromatography of Uroporphyrin Isomers
High Performance Liquid Chromatography of Uroporphyrin Isomers
复制标题
尿卟啉异构体的高效液相色谱法
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
C. Lim
中科院分区：
文献类型：
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作者：
J. Rideout;D. Wright;C. Lim
摘要采用反相高效液相色谱法，以乙酸铵缓冲液-乙腈为溶剂，在ODS-Hypersil柱上分离尿卟啉I、III异构体。考察了缓冲液浓度、pH值、有机改性剂比例及不同有机改性剂对拆分的影响。分离的最佳条件为：1M醋酸铵缓冲液中，乙腈浓度为12~13%，pH为5.10~5.20。该方法也能将尿卟啉I、III从II异构体中分离出来，但不能实现对尿卟啉III、IV的拆分。
Abstract The separation of uroporphyrin I and III isomers by reversed-phase high performance liquid chromatography on ODS-Hypersil with ammonium acetate buffer-acetonitrile solvent systems is described. The effects of buffer concentration, pH, organic modifier proportion and different organic modifiers on the resolution are studied. The optimum conditions for the separation were 12–13% acetonitrile in 1M ammonium acetate buffer pH 5.10–5.20. The method also separated uroporphyrin I and III from the II isomers but the resolution of uroporphyrin III and IV isomers was not achieved.