Virus-Delivered RNA Interference in Mouse Brain to Study Addiction-Related Behaviors
Virus-Delivered RNA Interference in Mouse Brain to Study Addiction-Related Behaviors
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DOI：
10.1007/978-1-60761-058-8_17
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Azouaou, Nourredine
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文献类型：
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作者：
Lasek, Amy W.;Azouaou, Nourredine
使用病毒载体将基因转移到特定的脑区域是在小鼠行为模型中确定基因功能的有力工具。我们已经采用慢病毒载体将小发夹RNA递送到与药物成瘾相关的行为相关的小鼠大脑区域，如延髓核和腹侧被盖区。表达小发夹RNA的病毒的递送导致通过RNA干扰持续的靶基因敲低。随后，在几周或几个月的时间里，测试小鼠对各种滥用药物的行为反应。在这里，我们描述了一种方法，立体定向交付慢病毒小鼠大脑。这种方法广泛适用于任何行为实验，其中特定基因在特定脑区的作用是要阐明的。
The use of viral vectors for gene transfer to specific brain regions is a powerful tool for determining gene function ill Mouse behavioral models. We have employed a lentiviral vector to deliver small-hairpin RNAs to areas Of Mouse brain implicated in behaviors relevant to drug addiction, such as the nucleus accumbens and ventral tegmental area. Delivery of virus expressing small-hairpin RNAs results in Sustained target gene knockdown by RNA interference. Mice call Subsequently be tested for behavioral responses to various drugs of abuse over the course of several weeks or months. Here we describe a method for stereotaxic delivery of lentivirus to mouse brain. This method is widely applicable to any behavioral experiment in which the role of a specific gene in a particular brain region is to be elucidated.