Identification of human neutrophils during experimentally induced inflammation in mice with transplanted CD34+ cells from human umbilical cord blood
Identification of human neutrophils during experimentally induced inflammation in mice with transplanted CD34+ cells from human umbilical cord blood
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DOI：
10.1532/ijh97.06040
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发表时间：
2006-10-01
影响因子：
2.1
通讯作者：
Yuo, Akira
中科院分区：
文献类型：
--
作者：
Doshi, Masaru;Koyanagi, Makoto;Yuo, Akira
非肥胖糖尿病/严重联合免疫缺陷/伽马链(NOG)小鼠是良好的异种移植受体，在评估人类造血干细胞(HSC)活性方面特别有价值。由于在这只小鼠体内发育的人类造血细胞主要是淋巴样细胞，而不是髓系细胞，因此在外周血中几乎检测不到中性粒细胞等成熟的人髓系细胞。我们证明，在NOG小鼠中，通过酵母多糖诱导的气囊炎症技术积聚的人中性粒细胞可以用荧光激活细胞分选器在移植了人脐血CD34(+)细胞的NOG小鼠中鉴定出来，这些细胞可能是包括HSC在内的造血祖细胞。我们的结果表明，具有趋化能力的人中性粒细胞可以在体内从人造血祖细胞分化而来，这表明我们的系统可能是评估人HSC活性的有用工具。
Nonobese diabetic/severe combined immunodeficiency/gamma chain (NOG) mice are excellent recipients for xenotransplantation and have been especially valuable for the evaluation of human hematopoietic stem cell (HSC) activities. Because human hematopoietic cells that developed in this mouse were mainly lymphoid cells and not myeloid cells, mature human myeloid cells such as neutrophils were hardly detectable in peripheral blood. We demonstrated that human neutrophils accumulated by means of a zymosan-induced air pouch inflammation technique could be identified with a fluorescence-activated cell sorter in NOG mice with transplanted CD34(+) cells from human umbilical cord blood, which were putative hematopoietic progenitor cells including HSC. Our results indicate that human neutrophils with a chemotactic capacity can develop from human hematopoietic progenitor cells in vivo, suggesting that our system may be a useful tool for the evaluation of human HSC activities.