Deuterium nuclear magnetic resonance spectroscopy and stable carbon isotope ratio analysis/mass spectrometry of certain monofloral honeys.
Deuterium nuclear magnetic resonance spectroscopy and stable carbon isotope ratio analysis/mass spectrometry of certain monofloral honeys.
复制标题
某些单花蜂蜜的氘核磁共振波谱和稳定碳同位素比分析/质谱分析。
DOI：
10.1093/jaoac/83.6.1401
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发表时间：
2000
影响因子：
1.6
通讯作者：
M. H. Merle
中科院分区：
文献类型：
--
作者：
S. Giraudon;M. Danzart;M. H. Merle
定量氘核磁共振光谱（NMR）已与稳定碳同位素比分析/质谱联用，以改进已添加到单花蜂蜜中的糖的检测。糖的13 C含量指示合成它们的植物的光合代谢的类型;氘含量更具有次生代谢和环境因素的特征。因此，蜂蜜和从蜂蜜中提取的蛋白质的13 C含量的测定可用于检测C4植物糖（甘蔗或玉米）的添加，但它不能揭示C3植物糖如甜菜糖的添加。氘核磁共振给出了一些单花蜂蜜的有用信息。对从蜂蜜发酵获得并通过蒸馏提取的乙醇进行NMR测量。乙醇的同位素组成表明其来源的糖的性质。对各种类型的单花蜂蜜进行了研究，用市售蜂蜜获得的结果证明了同位素分析的有用性，并需要编译真实蜂蜜的数据库以验证或确认某些结果。
Quantitative deuterium nuclear magnetic resonance spectroscopy (NMR) has been used in conjunction with stable carbon isotope ratio analysis/mass spectrometry to refine the detection of sugars that have been added to monofloral honeys. The 13C content of sugars indicates the type of photosynthetic metabolism of the plant that synthesized them; the deuterium content is more characteristic of secondary metabolism and of environmental factors. Consequently, determination of the 13C content of honeys and of proteins extracted from the honeys can be used to detect the addition of C4 plant sugars (cane or corn), but it does not reveal the addition of C3 plant sugars such as beet sugar. Deuterium NMR gives useful information for some monofloral honeys. NMR measurement is performed on ethanol obtained from fermentation of the honey and extracted by distillation. The isotopic composition of the ethanol indicates the nature of the sugars from which it was derived. Various types of monofloral honeys were studied, and the results obtained with commercially available honeys demonstrate the usefulness of isotopic analysis and the need to compile a database of authentic honeys to validate or affirm certain results.