Intersubgeneric hybridization between Glycine max and G. tomentella: production of F1, amphidiploid, BC1, BC2, BC3, and fertile soybean plants
Intersubgeneric hybridization between Glycine max and G. tomentella: production of F1, amphidiploid, BC1, BC2, BC3, and fertile soybean plants
复制标题
Glycine max 和 G. tomentella 之间的亚属间杂交：产生 F1、双二倍体、BC1、BC2、BC3 和可育大豆植株
DOI：
10.1007/s00122-015-2494-0
复制
发表时间：
2015
影响因子：
5.4
通讯作者：
R. Nelson
中科院分区：
文献类型：
--
作者：
R. Singh;R. Nelson
本文描述了从澳大利亚多年生野生大豆中解锁遗传宝藏以进行大豆改良的方法。摘要大约26种大豆亚种的遗传资源尚未被利用来拓宽大豆的遗传基础(Glycinmax；2n=2.40)。本研究的目的是为了在大豆育种中利用大豆亚属种质资源，开发利用大豆与大豆亚种杂交产生F1、双二倍体、Bc1、BC2、BC3和可育植株的方法。大豆品种与6个78-G染色体的材料杂交。绒毛和40号染色体各1个。Tomentella、银杏G.argyrea.宽叶木属。它们之所以被选中，是因为它们表现出对大豆锈病的抗性。我们成功地用大豆品种生产出了肥沃的大豆。‘Dwight’和78号染色体G。Pi 441001，而其他杂交种在F1或双二倍体期终止。F1种子在成熟之前就败育了，所以从19到21天的豆荚发育的种子在不同的培养基配方中进行无菌培养。研制了种子成熟培养和多胚代培养体系。将2n=259的F1植株移栽到温室盆栽中。两倍体(2n=9118)植株回交至‘德怀特’。对Bc1(2n=779)植株进行了组培繁殖，获得了43粒成熟的BC2F1种子。15个存活的BC_2F_1植株形态独特，不育，染色体数目为2n=256-59。BC3F_1植株的染色体数目为2n=440-49。衍生可育大豆于2008年首次在田间种植，目前正在通过材料转让协议对产量、对病原体和害虫的抗性以及耐盐性进行评估。
Key messageThis paper describes methods for unlocking genetic treasure from wild perennialGlycinespecies of Australia for soybean improvement.AbstractThe genetic resources of the ca. 26 species of the genusGlycinesubgenusGlycinehave not been exploited to broaden the genetic base of soybean (Glycine max; 2n= 40). The objectives of this study were to develop methods for producing F1, amphidiploid, BC1, BC2, BC3, and fertile soybean plants from crosses of soybean and the genusGlycinesubgenusGlycinespecies, in order to utilize the subgenusGlycinegermplasm in soybean breeding. Soybean cultivars were hybridized with six accessions of 78-chromosomeG. tomentellaas well as one accession each of 40-chromosomeG. tomentella,G. argyreaandG. latifolia. They were chosen because they exhibit resistance to soybean rust. We were successful in producing fertile soybean from soybean cv. ‘Dwight’ and 78-chromosomeG. tomentellaaccession PI 441001, while other hybrids were discontinued either at F1or amphidiploid stage. The F1seeds aborted prior to reaching maturity, so developing seeds from 19 to 21 day old pods were cultured aseptically in various media formulations. Seed maturation and multiple embryo generation media were developed. F1plants with shoots and roots (2n= 59) were transplanted to pots in greenhouse. Amphidiploid (2n= 118) plants were backcrossed to ‘Dwight’. BC1(2n= 79) plants were propagated through in vitro and 43 mature BC2F1seeds were harvested. Fifteen surviving BC2F1plants were morphologically distinct, sterile, and had chromosome numbers ranging 2n= 56–59. Chromosome numbers of the BC3F1plants ranged 2n= 40–49. Derived fertile soybeans were first planted in the field in 2008 and are being evaluated for yield, resistance to pathogens and pests and tolerance to salt through material transfer agreement.