Changes in esterase zymograms in the silkworm, Bombyx mori L., during development.
Changes in esterase zymograms in the silkworm, Bombyx mori L., during development.
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DOI：
10.1016/0022-1910(65)90184-8
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发表时间：
1965-08
影响因子：
2.2
通讯作者：
M. Eguchi;T. Sugimoto
中科院分区：
文献类型：
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作者：
M. Eguchi;T. Sugimoto
采用琼脂凝胶薄层电泳法对家蚕各组织的酯酶进行了研究。以β-乙酸萘酯为底物，萘酰重氮蓝B为偶联剂，检测了9条不同的酯酶条带。八个带向阳极迁移，而一个带向阴极迁移。与其他生物体一样，在观察到的模式中存在阶段和组织特异性。酶活性在发育过程中逐渐发生变化，从5龄后期到蛹期有较大的变化。用pH指示剂法测定了血、肠酯酶的底物和抑制特异性，并讨论了各组分的性质。
By means of thin layer electrophoresis in agar gel, esterases of various tissues of the silkworm were studied. Nine different esterase bands were detected with β-naphthyl acetate as the substrate and naphthanil diazoblue B as coupler. Eight bands migrated towards the anode, while one band migrated towards the cathode. There is a stage and tissue specificity in the patterns observed, as in other organisms. Gradual changes in enzyme activity occurred during development, and there were great shifts from the late period of the fifth instar to the pupal stage. Substrate and inhibition specificities of the blood and intestine esterases were tested by pH indicator method, and the nature of each component was discussed.