Complex Formation of RNA Silencing Proteins in the Perinuclear Region of Neurospora crassa
Complex Formation of RNA Silencing Proteins in the Perinuclear Region of Neurospora crassa
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DOI：
10.1534/genetics.115.174623
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发表时间：
2015-04-01
期刊：
影响因子：
3.3
通讯作者：
Shiu, Patrick K. T.
中科院分区：
文献类型：
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作者：
Decker, Logan M.;Boone, Erin C.;Shiu, Patrick K. T.
在脉孢菌中，减数分裂期间不配对的基因通过未配对DNA（MSUD）的减数分裂沉默而被靶向。在这里，我们的双分子荧光互补（BiFC）研究表明，RNA指导的RNA聚合酶，Dicer，Argonaute等在核周区域形成沉默复合物，其中大多数之间存在密切的相互作用。我们还表明SAD-2可能是该组装的锚。
In Neurospora, genes not paired during meiosis are targeted by meiotic silencing by unpaired DNA (MSUD). Here, our bimolecular fluorescence complementation (BiFC) study suggests that RNA-directed RNA polymerase, Dicer, Argonaute, and others form a silencing complex in the perinuclear region, with intimate interactions among the majority of them. We have also shown that SAD-2 is likely the anchor for this assembly.