Effect of Delivery Mode and Nutrition on Gut Microbiota in Neonates
Effect of Delivery Mode and Nutrition on Gut Microbiota in Neonates
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DOI：
10.1159/000496427
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发表时间：
2019-01-01
影响因子：
3.9
通讯作者：
Kaneko, Kazunari
中科院分区：
文献类型：
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作者：
Akagawa, Shohei;Tsuji, Shoji;Kaneko, Kazunari
背景/目标：新生儿的分娩方式（阴道或剖宫产）和喂养类型（母乳喂养或配方奶粉喂养）被认为是肠道微生物群发育的最具影响力的因素。目的：本研究调查了富含益生元的母乳对克服肠道微生物群失调的影响。方法：从36名健康的日本新生儿的粪便样本，在4天和1个月的年龄，并分为4组的基础上的交付方式和喂养类型。使用16 S rRNA测序评估肠道微生物群组成和细菌多样性。结果：4日龄时，阴道分娩新生儿的细菌多样性明显高于剖宫产新生儿。阴道分娩新生儿中类杆菌和肠杆菌的比例过高（p = 0.0031和p = 0.011），而剖腹产新生儿中杆菌和乳酸杆菌的比例过高（p = 0.012和p = 0.0016）。然而，在1月龄时，各组之间的细菌多样性和细菌相对丰度差异不大。结论：剖腹产似乎减少了新生儿肠道微生物群的多样性，导致生态失调，但这在1个月大时改善到阴道分娩婴儿的同等水平。因此，母乳喂养，即使是短期的，也可能改善新生儿肠道生态失调。(c)2019 S. Karger AG，巴塞尔
Background/Aims: The mode of delivery (vaginal or cesarean section) and feeding type (breastfeeding or formula feeding) of neonates are considered the most influential factors in the development of gut microbiota. Objectives: This study investigated the effect of prebiotic-rich breast milk on overcoming gut microbiota dysbiosis. Method: Stool samples from 36 healthy Japanese neonates were obtained at 4 days and 1 month of age, and divided into 4 groups based on mode of delivery and feeding type. The gut microbiota composition and bacterial diversity were assessed using 16S rRNA sequencing. Results: At 4 days old, vaginally delivered neonates had a significantly higher diversity of bacteria than those born by cesarean section. Bacteroidales and Enterobacteriales were overrepresented in vaginally delivered neonates (p = 0.0031 and p = 0.011), while Bacillales and Lactobacillales were overrepresented in caesarean section delivered neonates (p = 0.012 and p = 0.0016). However, there was little difference in bacterial diversity and bacterial relative abundance at 1 month of age between groups. Conclusions: Cesarean section delivery appeared to reduce the diversity of neonate gut microbiota, resulting in dysbiosis, but this improved to the equivalent level seen in vaginally delivered infants by 1 month of age. Breastfeeding, even for short periods, may therefore improve neonate gut dysbiosis. (c) 2019 S. Karger AG, Basel