The effects of drug delivery via hydrophilic acrylic (hydrogel) intraocular lens systems on the epithelial cells in culture
The effects of drug delivery via hydrophilic acrylic (hydrogel) intraocular lens systems on the epithelial cells in culture
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DOI：
10.3928/1542-8877-20050901-07
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发表时间：
2005-09-01
影响因子：
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通讯作者：
Clark, JI
中科院分区：
文献类型：
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作者：
Matsushima, H;Mukai, K;Clark, JI
背景与目的：继发性后囊下混浊仍然是超声乳化术后最重要的并发症之一。材料与方法：本研究旨在评估药物通过亲水性丙烯酸（水凝胶）人工透镜（IOL）系统在体外的抑制作用。从白化病兔收集透镜上皮细胞。制备了以下7组水凝胶IOL：未处理IOL和用双氯芬酸钠、曲尼司特、丝裂霉素C、秋水仙碱、5-氟尿嘧啶和乙二胺四乙酸浸润的IOL。将IOL固定在细胞培养插入物上;然后将其在含有培养的透镜上皮细胞的最低必需培养基中浸泡和孵育。结果：对照组透镜上皮细胞在透镜表面及胶原膜上均有粘附。然而，只有轻微的细胞粘附被发现在IOL的表面上和胶原膜上的治疗IOL groups.CONCLUSION：使用水凝胶IOL渗透药物与抑制后囊下混浊在体外。
BACKGROUND AND OBJECTIVE: Secondary posterior subcapsular opacification is still among the most important complications after phacoemulsification.MATERIALS AND METHODS: This study was designed to assess the inhibitory effects of drugs delivered via hydrophilic acrylic (hydrogel) intraocular lens (IOL) systems in vitro. Lens epithelial cells were collected from albino rabbits. The following seven groups of hydrogel IOLs were prepared: untreated IOLs and IOLs infiltrated with diclofenac sodium, tranilast, mitomycin C, colchicines, 5-fluorouracil, and ethylenediaminetetraacetic acid. The IOLs were fixed to a Cell Culture Insert; they were then bathed and incubated in minimum essential medium containing cultured lens epithelial cells. Subsequently, a comparative analysis of the cells adhering to the collagen membrane and the lens surfaces was conducted.RESULTS: Adhesion of lens epithelial cells to the lens surfaces and the collagen membrane was observed in the control group. However, only slight cellular adhesion was found on the surfaces of the IOLs and on the collagen membrane in the treated IOL groups.CONCLUSION: Use of hydrogel IOLs infiltrated with drugs was associated with inhibition of posterior subcapsular opacification in vitro.