MATN and LAPTM are parts of larger transcription units produced by intergenic splicing:: Intergenic splicing may be a common phenomenon
MATN and LAPTM are parts of larger transcription units produced by intergenic splicing:: Intergenic splicing may be a common phenomenon
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DOI：
10.1093/dnares/dsi017
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发表时间：
2005-10-31
期刊：
影响因子：
4.1
通讯作者：
Ikegawa, Shiro
中科院分区：
文献类型：
--
作者：
Maeda, Koichi;Horikoshi, Taizo;Ikegawa, Shiro
基因间剪接，即来自不同基因的外显子的连接，在哺乳动物中很少被观察到。虽然基质蛋白（MATN）和溶酶体相关蛋白跨膜（LAPTM）基因包含不同的基因家族，但我们已经证明了两组家族基因之间的基因间剪接，即基质蛋白-3（MATN 3）和溶酶体相关蛋白跨膜4 α（LAPTM 4 A），以及基质蛋白-2（MATN 2）和溶酶体相关蛋白跨膜4）3（LAPTM 4 B）。MATN-LAPTM杂合转录物的表达模式和亚细胞定位与原始基因的表达模式和亚细胞定位不同，表明产物具有独特的功能。我们的观察结果表明，基因间剪接是一种常见的和良好的调节现象，并强调在定义基因（转录单位）的根本挑战。鉴于这些发现，人类基因组中的基因数量可能比目前估计的要少。
Intergenic splicing, the joining of exons from separate genes, has been observed only rarely in mammals. While the matrilin (MATN) and lysosomal-associated protein transmembrane (LAPTM genes comprise distinct gene families, we have demonstrated intergenic splicing between two sets of family genes, the matrilin-3 (MATN3) and lysosomal-associated protein transmembrane 4 alpha (LAPTM4A), and the matrilin-2 (MATN2) and lysosomal-associated protein transmembrane 4)3 (LAPTM4B). The expression pattern and sub-cellular localization of the MATN-LAPTM hybrid transcripts differ from those of the original genes, suggesting unique functions for the products. Our observations indicate that intergenic splicing is a common and well-regulated phenomenon and underscore the fundamental challenges in defining the gene (transcriptional unit). Given these findings, the number of gene in the human genome may be smaller than present estimates suggest.