Serum klotho protein levels and their correlations with the progression of type 2 diabetes mellitus
Serum klotho protein levels and their correlations with the progression of type 2 diabetes mellitus
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DOI：
10.1016/j.jdiacomp.2016.11.008
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发表时间：
2017-03-01
影响因子：
3
通讯作者：
Xu, Ying
中科院分区：
文献类型：
--
作者：
Nie, Fang;Wu, Dongming;Xu, Ying
目的：探讨血清 α-Klotho 和 β-Klotho 水平与 2 型糖尿病 (T2DM) 进展的关系。方法：我们评估了 106 名健康对照者和 261 例伴或不伴糖尿病并发症的 T2DM 病例（范围：45-84 岁）。采用酶联免疫吸附法分析血清α-Klotho和β-Klotho水平。结果：与健康对照相比，患有或不患有糖尿病并发症的T2DM患者的α-Klotho和β-Klotho水平显着降低（P < 0.05）。此外，与正常白蛋白尿组相比，微量白蛋白尿组和大量白蛋白尿组的 α-Klotho 水平较低。然而，β-Klotho 水平仅在大量白蛋白尿组中较低（P < 0.05）。多元线性回归分析显示，ot-Klotho和β-Klotho水平与肌酐清除率呈正相关，与尿白蛋白与肌酐比值以及随机采样的血清肌酐、血尿素氮和血糖水平呈负相关。此外，α-Klotho和β-Klotho水平在T2DM患者中呈正相关（r = 0.693，P < 0.001）。结论：α-Klotho和β-Klotho水平在T2DM患者中下调。因此，这些蛋白可能参与了糖尿病的病理机制，并且α-Klotho和α-Klotho水平的正相关表明它们在T2DM中可能具有相似的机制。 (C) 2017 Elsevier Inc. 保留所有权利。
Aim: To investigate the associations of serum alpha-Klotho and beta-Klotho levels with type 2 diabetes mellitus (T2DM) progression.Methods: We evaluated 106 healthy controls and 261 cases of T2DM with or without diabetic complications (range: 45-84 years). Serum alpha-Klotho and beta-Klotho levels were analyzed using enzyme-linked immunosorbent assays.Results: Compared to the healthy controls, alpha-Klotho and beta-Klotho levels were significantly lower among patients with T2DM and with or without diabetic complications (P < 0.05). Furthermore, alpha-Klotho levels were lower in the microalbuminuric and macroalbuminuric groups, compared to the normoalbuminuric group. However, beta-Klotho levels were only lower in the macroalbuminuric group (P < 0.05). Multiple linear regression analyses revealed that ot-Klotho and beta-Klotho levels were positively correlated with the creatinine clearance rate, and negatively correlated with the urinary albumin to creatinine ratio and randomly sampled serum levels of creatinine, blood urea nitrogen, and blood glucose. Moreover, alpha-Klotho and beta-Klotho levels were positively correlated among patients with T2DM (r = 0.693, P < 0.001).Conclusions: Serum levels of alpha-Klotho and beta-Klotho are down-regulated in patients with T2DM. Thus, these proteins may participate in the pathological mechanism of diabetes, and the positive correlation of alpha-Klotho and alpha-Klotho levels indicates that they might have similar mechanisms in T2DM. (C) 2017 Elsevier Inc. All rights reserved.