Differences in ischemic lesion evolution in different rat strains using diffusion and perfusion imaging
Differences in ischemic lesion evolution in different rat strains using diffusion and perfusion imaging
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DOI：
10.1161/01.str.0000177486.85508.4d
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发表时间：
2005-09-01
期刊：
影响因子：
8.3
通讯作者：
Fisher, M
中科院分区：
文献类型：
--
作者：
Bardutzky, J;Shen, Q;Fisher, M
背景和目的：大鼠大脑中动脉闭塞(MCAO)后缺血时间演变的品系间差异可能会对实验性卒中的研究结果产生很大影响。在两个常用的大鼠(SD和Wistar-京都[WK])上，我们用扩散成像和灌注成像研究了MCAO后脑缺血的时空演变。方法连续测量脑血流量(CBF)和表观扩散系数(ADC)至MCAO后210min。结果SD大鼠大脑中动脉ADC损伤体积在MCAO后120min迅速增加，3h后基本停止生长，而WK大鼠ADC损伤体积在整个210min内逐渐增加，并在所有时间点均显著缩小(P<；0.05)。两组患者的异常灌注量与TTC定义的梗塞面积高度相关。WK大鼠脑缺血90min时异常灌注量明显大于弥散异常体积(P<；0.001)，而SD大鼠仅在45min时弥散/灌流失配显著(P<；0.001)。ADC-CBF散点图分析显示，在重度(正常的20%)和中度(正常的21%至40%)CBF下降的像素中，WK大鼠的ADC随时间的下降速度较慢且不那么强劲。结论--本研究显示SD大鼠和WK大鼠在急性缺血性病变演变方面存在显著差异。在评估大鼠MCAO模型中缺血性损伤演变的新治疗方法时，必须考虑这些菌株间的差异。
Background and Purpose - Interstrain differences in the temporal evolution of ischemia after middle cerebral artery occlusion (MCAO) in rats may considerably influence the results of experimental stroke research. We investigated, in 2 commonly used rat strains (Sprague-Dawley [SD] and Wistar-Kyoto [WK]), the spatiotemporal evolution of ischemia after permanent suture MCAO using diffusion and perfusion imaging.Methods - Serial measurements of quantitative cerebral blood flow (CBF) and apparent diffusion coefficient ( ADC) were performed up to 210 min after MCAO. Lesion volumes were calculated by using previously established viability thresholds and correlated with infarct volume defined by 2,3,5-triphenyltetrazolium chloride staining 24 hours after MCAO.Results - While the ADC-derived lesion volume increased rapidly during the first 120 min after MCAO and essentially stopped growing after 3 hours in SD rats, ADC lesion in WK rats increased progressively during the entire 210-min period and was significantly smaller at all time points (P < 0.05). The abnormal perfusion volume correlated highly with the TTC-defined infarct size in both groups. In WK rats, the abnormal perfusion volume was significantly larger than the abnormal diffusion volume up to 90 min after MCAO (P < 0.001), whereas the diffusion/perfusion mismatch was significant (P < 0.001) only at 45 min in SD rats. ADC - CBF scatterplots analysis revealed a slower and less robust ADC decline over time in WK rats in pixels with severe (< 20% of normal) and moderate ( 21 to 40% of normal) CBF reduction.Conclusions - This study demonstrated substantial differences in acute ischemic lesion evolution between SD and WK rats. These interstrain variations must be taken into account when assessing new therapeutic approaches on ischemic lesion evolution in the rat MCAO model.