Role of dorsal hippocampus in acquisition, consolidation and retrieval of rat's passive avoidance response: A tetrodotoxin functional inactivation study
Role of dorsal hippocampus in acquisition, consolidation and retrieval of rat's passive avoidance response: A tetrodotoxin functional inactivation study
复制标题
DOI：
10.1016/0006-8993(96)00427-1
复制
发表时间：
1996-08-19
期刊：
影响因子：
2.9
通讯作者：
Tassoni, G
中科院分区：
文献类型：
--
作者：
Lorenzini, CA;Baldi, E;Tassoni, G
通过局部给药河豚毒素（TTX），获得了大鼠背海马（DH）完全可逆的功能失活，以确定该结构在条件被动回避反应（PAR）记忆中的作用。在实验1中，对永久插管动物，分别在PAR获取前1小时、PAR获取后立即和PAR获取前1小时在DH单侧或双侧注射TTX （1.0 μ l生理盐水中10 ng）或生理盐水（1.0 μ l），每次均在获取试验后48小时进行。结果表明，获取前和检索前的DH单侧或双侧阻断均导致显著的PAR保留损伤，而获取后仅双侧阻断决定PAR保留损伤。在实验2中，在三组不同的大鼠身上，在全身氯胺酮麻醉（100 mg/kg）下，在不同的获取后延迟（0.25、1.5、6 h）给药TTX （10 ng / 1 μ l生理盐水）。治疗后48小时的检索测试显示，仅在获取后0.25或1.5小时进行双侧DH阻断时才会导致PAR损伤。结果表明，在PAR印痕的获取、巩固和检索过程中，DH具有明确的助记作用。实验证据讨论了相关的其他报告和DH连接与内侧隔区和杏仁核。
By means of local administration of tetrodotoxin (TTX) fully reversible functional inactivation of rat's dorsal hippocampus (DH) was obtained in order to define the role of this structure in the memorization of a conditioned passive avoidance response (PAR). In Experiment 1, on permanently cannulated animals, TTX (10 ng in 1.0 mu l saline) or saline (1.0 mu l) was injected uni- or bilaterally in the DH, respectively 1 h before PAR acquisition, immediately after PAR acquisition, and 1 h before PAR retrieval, always performed 48 h after the acquisition trial. It was shown that both pre-acquisition and pre-retrieval DH uni- or bilateral blockades were followed by significant PAR retention impairment, while in post-acquisition only the bilateral blockade determined PAR retention impairment. In Experiment 2, on three different groups of rats, TTX (10 ng in 1 mu l saline) was bilaterally administered, under general ketamine anesthesia (100 mg/kg), into the DH at different post-acquisition delays (0.25, 1.5, 6 h). Retrieval testing, 48 h after treatment, showed that post-acquisition bilateral DH blockade caused PAR impairment only when performed 0.25 or 1.5 h after acquisition. The results indicate a well defined mnemonic role of DH during the acquisition, consolidation and retrieval of PAR engram. The experimental evidence is discussed in relation to other reports and to DH connectivity with the medial septal area and the amygdala.