Tissue Factor and Its Measurement in Whole Blood, Plasma, and Microparticles
Tissue Factor and Its Measurement in Whole Blood, Plasma, and Microparticles
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DOI：
10.1055/s-0030-1267040
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发表时间：
2010-11-01
影响因子：
5.7
通讯作者：
Mackman, Nigel
中科院分区：
文献类型：
--
作者：
Key, Nigel S.;Mackman, Nigel
组织因子(Tf)是一种跨膜蛋白，在与凝血因子VII/VIIa接触后启动凝血。最近的实验证据，特别是来自动物模型的证据表明，循环中的转铁蛋白在血栓形成中起着重要的作用。这使得人们对全血和无细胞血浆中转铁蛋白的测量产生了越来越大的兴趣，在无细胞血浆中，功能性转铁蛋白被携带在细胞来源的微粒上。在这篇综述中，我们讨论了已发表的用于检测循环TF抗原和活性的各种检测方法。我们对影响结果及其解释的一些关键的分析前和分析变量进行了评论。
Tissue factor (TF) is a transmembrane protein that initiates coagulation following contact with factor VII/VIIa. Recent experimental evidence, in particular from animal models, suggests an important role for circulating TF in thrombosis. This has led to a growing interest in the measurement of TF in whole blood and in cell-free plasma, where functionally active TF is carried on cell-derived microparticles. in this review, we address the range of assays for measuring circulating TF antigen and activity that have been published. We comment on some of the crucial preanalytical and analytical variables that influence the results and their interpretation.