Plant regeneration from protoplasts of peppermint (Mentha piperita L.)
Plant regeneration from protoplasts of peppermint (Mentha piperita L.)
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薄荷（Mentha Piperita L.）原生质体的植物再生
DOI：
10.1007/bf00233057
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发表时间：
1993
影响因子：
6.2
通讯作者：
Yoshio N. Ito
中科院分区：
文献类型：
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作者：
Hiroshi Sato;S. Enomoto;S. Oka;K. Hosomi;Yoshio N. Ito
酶法分离薄荷（Mentha piperitaL.）在含有1 mg/l NAA、0.4mg/l BA、0.5%蔗糖、0.5M甘露醇和0.1%Gelrite的改良B5培养基（第一培养基）中培养。在25°C黑暗中培养30 d后，原生质体形成由约100个细胞组成的菌落。将Gelrite培养基块转移到液体培养基中以促进进一步生长。转移到0.2%Gelrite固化培养基（与第一培养基相同的组分）中的0.5mm菌落在光照下孵育形成绿色愈伤组织（1-2 mm）。将绿色愈伤组织转移到分化培养基（B5，0.1 mg/l NAA，5 mg/l BA，2%蔗糖，0.2 M甘露醇，0.2%Gelrite）上，3-4周后产生芽。在不含激素的B5培养基中生根后，回收整个植株。
Enzymatically isolated leaf-derived protoplasts of peppermint (Mentha piperitaL.) were cultured in modified B5 medium containing 1 mg/l NAA, 0.4 mg/l BA, 0.5% sucrose, 0.5 M mannitol and 0.1% Gelrite (first medium). After 30 d culture at 25°C in the dark, protoplasts formed colonies consisting of about 100 cells. Gelrite medium blocks were transferred into liquid medium to promote further growth. Colonies of 0.5 mm transferred to 0.2% Gelrite solidified medium (same components as first medium) formed green calli (1–2 mm) under incubation in the light. Green calli transferred to differentiation medium (B5, 0.1 mg/l NAA, 5 mg/l BA, 2% sucrose, 0.2 M mannitol, 0.2% Gelrite) developed shoot buds after 3–4 weeks. Whole plants were recovered following rooting of shoots in B5 medium without hormones.