[Expressions of Renin, angiotensin converting enzyme, angiotensin receptor 1, and angiotensin receptor 2 in synovial tissue of osteoarthritis at different stages].
[Expressions of Renin, angiotensin converting enzyme, angiotensin receptor 1, and angiotensin receptor 2 in synovial tissue of osteoarthritis at different stages].
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DOI：
10.7507/1002-1892.201904065
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发表时间：
2020-03
影响因子：
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通讯作者：
Yuangang Wu;Y. Zeng;Mingyang Li;Yuan Liu;Jing Yang;B. Shen
中科院分区：
文献类型：
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作者：
Yuangang Wu;Y. Zeng;Mingyang Li;Yuan Liu;Jing Yang;B. Shen
目的探讨骨关节炎（OA）滑膜组织中肾素（Renin）、血管紧张素转换酶（ACE）、血管紧张素受体1（AT 1 R）、血管紧张素受体2（AT 2 R）的表达。方法选择2018年1月至2018年12月因创伤行膝关节截肢或全膝关节置换术治疗OA的患者。其中，32例符合选择标准的患者被纳入研究。根据膝关节Kelling-Lawrence（K-L）X线分型分为正常滑膜组（A组，n=9）、中度骨性关节炎滑膜组（B组，n=11）和晚期骨性关节炎滑膜组（C组，n=12）。采用实时荧光定量PCR（qRT-PCR）和Western blot检测各组大鼠肾组织中肾素、血管紧张素转换酶（ACE）、血管紧张素1受体（AT 1 R）和血管紧张素2受体（AT 2 R）mRNA和蛋白的相对表达。结果与A组相比，B组和C组大鼠肾组织中肾素、血管紧张素转换酶（ACE）和血管紧张素转换酶1受体（AT 1 R）mRNA和蛋白的相对表达量均显著升高（P<0. 05）。C组血管紧张素转换酶（ACE）、血管紧张素转换酶1受体（AT 1 R）mRNA和蛋白的相对表达量及肾素蛋白的相对表达量均显著高于B组（P<0.05）。B组和C组AT 2 R mRNA和蛋白的相对表达量均低于A组（P<0.05），C组低于B组（P<0.05）。结论随着K-L水平的升高，骨关节炎滑膜组织中Renin、ACE和AT 1 R的表达明显增加，AT 2 R的表达降低。肾素、ACE、AT 1 R、AT 2 R与OA的发生发展有一定的相关性。
Objective To study the expressions of Renin, angiotensin converting enzyme (ACE), angiotensin receptor 1 (AT1R), and AT2R in synovial tissue of osteoarthritis (OA) at different stages. Methods The patients who were treated with upper knee amputation because of trauma or total knee arthroplasty for OA between January 2018 and December 2018 were enrolled. Among them, 32 patients who met the selection criteria were included in the study. According to the Kellgren-Lawrence (K-L) X-ray classification, they were allocated to normal synovial group (group A, n=9), moderate OA synovial group (group B, n=11, K-L level 3), and advanced OA synovial group (group C, n=12, K-L level 4). The relative expressions of Renin, ACE, AT1R, and AT2R mRNAs and proteins were detected by real-time fluorescence quantitative PCR (qRT-PCR) and Western blot. Results The relative expressions of Renin, ACE, and AT1R mRNAs and proteins were significantly higher in group B and group C than in group A ( P<0.05). The relative expressions of ACE and AT1R mRNAs and proteins and Renin protein were significantly higher in group C than in group B ( P<0.05). However, the relative expressions of AT2R mRNA and protein were lower in group B and group C than in group A ( P<0.05), and in group C than in group B ( P<0.05). Conclusion The expressions of Renin, ACE, and AT1R in synovial tissue of osteoarthritis significantly increase as the K-L level increased, and the expression of AT2R decreases. Renin, ACE, AT1R, and AT2R have a certain degree of correlation with the development of OA.