Immune response gene function correlates with the expression of an Ia antigen. I. Preferential association of certain Ae and E alpha chains results in a quantitative deficiency in expression of an Ae:E alpha complex
Immune response gene function correlates with the expression of an Ia antigen. I. Preferential association of certain Ae and E alpha chains results in a quantitative deficiency in expression of an Ae:E alpha complex
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免疫应答基因功能与 Ia 抗原的表达相关。
DOI：
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发表时间：
1982
影响因子：
15.3
通讯作者：
Patricia P. Jones
中科院分区：
文献类型：
--
作者：
J. McNicholas;D. Murphy;L. Matis;R. Schwartz;E. Lerner;C. Janeway;Patricia P. Jones
这些研究受到了随附论文（19）中报告的观察结果的刺激，即与来自其他Ia.7+单倍型的I-E亚区不同，IEu不能与F1小鼠中的I-Ak或I-As相互作用以允许对抗原（鸽子细胞色素c）的应答。进行血清学和生物化学分析，以确定这些F1小鼠的细胞是否表达Ak，se：E α复合物，该复合物应作为T细胞识别抗原呈递细胞上的鸽细胞色素c的限制性元件。使用Y-17单克隆抗体，其识别某些Ae：E α复合物上的组合或构象决定簇Ia.m44，我们能够区分细胞表面上的Aue：Eu α和Ab，k，se：Eu α复合物。虽然与Y-17的补体依赖性微细胞毒性未能检测到Ab，k，se：Eu α复合物从适当的F1小鼠的细胞，这些分子被检测到的定量吸收和定量免疫荧光研究。然而，发现Ab，k，se：Eu α复合物的水平仅为纯合I-Ab，I-Ek; I-Ak，I-Ek;和I-As，I-Ek细胞表达水平的七分之一到八分之一。二维聚丙烯酰胺凝胶电泳分析的结果表明，Ab，k，se：Eu α复合物的低水平的表达是Aue和Eu α链在F1细胞中彼此优先关联的结果。如下面的论文（19）所示，Ake：Eu α和Ase：Eu α复合物表达的定量缺陷导致抗原呈递细胞功能的相应缺陷，从而提供了Ia抗原代表Ir基因产物的有力证据。
These studies were stimulated by the observation, reported in the accompanying paper (19), that IEu failed to interact with I-Ak or I-As in F1 mice to allow a response to the antigen, pigeon cytochrome c, unlike I-E subregions derived from other Ia.7+ haplotypes. Serological and biochemical analyses were performed to determine whether or not cells from these F1 mice express the Ak,se:E alpha complexes that should function as restriction elements for T cell recognition of pigeon cytochrome c on antigen-presenting cells. Using the Y-17 monoclonal antibody, which recognizes the combinatorial or conformational determinant Ia.m44 on certain Ae:E alpha complexes, we were able to distinguish between Aue:Eu alpha and Ab,k,se:Eu alpha complexes on cell surfaces. Although complement-dependent microcytotoxicity with Y-17 failed to detect Ab,k,se:Eu alpha complexes on cells from appropriate F1 mice, these molecules were detected by both quantitative absorption and quantitative immunofluorescence studies. However, Ab,k,se:Eu alpha complexes were found to be present at levels only one-seventh to one-eighth the levels expressed by homozygous I-Ab, I-Ek; I-Ak, I-Ek; and I-As, I-Ek cells. The results of two-dimensional polyacrylamide gel electrophoresis analyses suggest that the low levels of expression of Ab,k,se:Eu alpha complexes are a consequence of the preferential association of Aue and Eu alpha chains with each other in the F1 cells. As will be shown in the following paper (19), the quantitative deficiency in the expression of Ake:Eu alpha and Ase:Eu alpha complexes results in a corresponding defect in antigen-presenting cell function, thus providing strong evidence that Ia antigens represent products of Ir genes.
DOI：
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发表时间：
1980
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Moosic,JP;Nilson,A;Hammerling,GJ;McKean,DJ
通讯作者：
McKean,DJ
I 区基因座之间的相互作用影响细胞表面 Ia 抗原的表达。
DOI：
10.1073/pnas.77.9.5404
发表时间：
1980
影响因子：
11.1
作者：
Murphy,DB;Jones,PP;Loken,MR;McDevitt,HO
通讯作者：
McDevitt,HO
与 H-2 复合物 I-A 亚区产物相关的新 B 细胞分化抗原的结构分析。
DOI：
10.1073/pnas.78.7.4525
发表时间：
1981
影响因子：
11.1
作者：
Huber,BT;Jones,PP;Thorley-Lawson,D
通讯作者：
Thorley-Lawson,D