Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy: possible involvement of periostin
Overexpression of CD163 in vitreous and fibrovascular membranes of patients with proliferative diabetic retinopathy: possible involvement of periostin
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DOI：
10.1136/bjophthalmol-2014-305321
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发表时间：
2015-04-01
影响因子：
4.1
通讯作者：
Ishibashil, Tatsuro
中科院分区：
文献类型：
--
作者：
Kobayashi, Yoshiyuki;Yoshida, Shigeo;Ishibashil, Tatsuro
目的探讨增生性糖尿病视网膜病变（proliferative diabetic retinopathy，PDR）患者视网膜前纤维血管膜（preretinal fibrovascular membrane，FVMs）的形成与M2巨噬细胞的特异性标志物CD 163的关系。增生性玻璃体视网膜病变18例20眼，非糖尿病性眼病54例56眼（对照组）。应用免疫组化方法检测PDR患者手术切除的FVM和特发性视网膜前膜（ERM）中CD 68、CD 163和periostin的表达。结果PDR患者玻璃体中sCD 163和periostin的浓度显著高于非糖尿病对照组（P < 0.05）。
Aim To determine whether CD163, a specific marker for M2 macrophages, is involved in the formation of preretinal fibrovascular membranes (FVMs) present in eyes with proliferative diabetic retinopathy (PDR).Methods We measured the levels of soluble (s)CD163, periostin and vascular endothelial growth factor by sandwich ELISA in vitreous samples from 74 eyes of 62 patients with PDR, 20 eyes of 18 patients with proliferative vitreoretinopathy, and 56 eyes of 54 patients with non-diabetic ocular diseases (control group). Immunohistochemical analyses were performed to determine the expressions of CD68, CD163 and periostin in the surgically resected FVMs and idiopathic epiretinal membranes (ERMs).Results The concentrations of sCD163 and periostin in the vitreous were significantly higher in patients with PDR than in non-diabetic controls (p