Detection of intensity change points in time-resolved single-molecule measurements
Detection of intensity change points in time-resolved single-molecule measurements
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DOI：
10.1021/jp0467548
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发表时间：
2005-01-13
影响因子：
3.3
通讯作者：
Yang, H
中科院分区：
文献类型：
--
作者：
Watkins, LP;Yang, H
我们提出了一种分析表现出离散强度跳跃的光学单分子发射数据的方法。这种新方法使用广义似然比测试，根据单个光子到达时间确定强度变化点的位置。该测试递归地应用于整个单分子强度轨迹，从而找到每个变化点。然后使用期望最大化聚类和贝叶斯信息准则来准确确定系统可访问的真实状态数。该过程允许严格和定量地确定强度变化点，而不受分箱和阈值处理产生的人为时间分辨率限制。
We present a method for the analysis of optical single molecule emission data that exhibit discrete intensity jumps. This new method uses a generalized likelihood ratio test that determines the location of an intensity change point based on individual photon arrival times. This test is applied recursively to an entire single molecule intensity trajectory, thus finding each change points. Expectation-maximization clustering and the Bayesian information criterion is then used for accurate determination of the true number of states accessible to the system. This procedure allows rigorous and quantitative determination of intensity change points without the artificial time resolution limitations that arise from binning and thresholding.