Nuclear pore complex is able to transport macromolecules with diameters of ∼39 nm
Nuclear pore complex is able to transport macromolecules with diameters of ∼39 nm
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DOI：
10.1091/mbc.01-06-0308
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发表时间：
2002-02-01
影响因子：
3.3
通讯作者：
Kann, M
中科院分区：
文献类型：
--
作者：
Panté, N;Kann, M
大分子在细胞核和细胞质之间的双向转运通过信号介导的机制通过核孔复合物（NPC）发生，该机制由驻留在转运分子或“货物”上的靶向信号（NLS）指导。“核转运在靶向信号与可溶性细胞受体相互作用后开始。在胞质溶胶中形成货物-受体复合物后，该复合物穿过NPC。在这里，我们使用不同大小的金颗粒与货物受体复合物涂层，以精确地确定有多大的大分子通过信号介导的运输机制可以通过NPC。我们发现直径接近39 nm的货物受体金复合物可以被NPC转运。这意味着比假定的26 nm的功能性NPC直径大得多的大分子可以被转运到核质中。直径为32和36 nm的完整人B型肝炎病毒核衣壳能够穿过核孔而不解体，这一观察结果支持了这一发现的生理相关性。
Bidirectional transport of macromolecules between the nucleus and the cytoplasm occurs through the nuclear pore complexes (NPCs) by a signal-mediated mechanism that is directed by targeting signals (NLSs) residing on the transported molecules or "cargoes." Nuclear transport starts after interaction of the targeting signal with soluble cellular receptors. After the formation of the cargo-receptor complex in the cytosol, this complex crosses the NPC. Herein, we use gold particles of various sizes coated with cargo-receptor complexes to determine precisely how large macromolecules crossing the NPC by the signal-mediated transport mechanism could be. We found that cargo-receptor-gold complexes with diameter close to 39 nm could be translocated by the NPC. This implies that macromolecules much larger than the assumed functional NPC diameter of 26 nm can be transported into the karyoplasm. The physiological relevance of this finding was supported by the observation that intact nucleocapsids of human hepatitis B virus with diameters of 32 and 36 nm are able to cross the nuclear pore without disassembly.