Quantitative analysis of ascorbic acid and dehydroascorbic acid by high-performance liquid chromatography.
Quantitative analysis of ascorbic acid and dehydroascorbic acid by high-performance liquid chromatography.
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采用高效液相色谱法定量分析抗坏血酸和脱氢抗坏血酸。
DOI：
10.1016/0003-2697(81)90464-4
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发表时间：
1981
影响因子：
2.9
通讯作者：
Nahrwold,DL
中科院分区：
文献类型：
--
作者：
Rose,RC;Nahrwold,DL
采用高效液相色谱-分光光度法分离和定量了抗坏血酸和脱氢抗坏血酸。这些维生素C的成分在licrosorb - nh2柱上分离。该技术能够定量地跟踪抗坏血酸氧化为脱氢抗坏血酸和反向还原。该技术被证明适用于测定生物样品、食品和药物维生素制剂中的维生素C。
High-performance liquid chromatography with spectrophotometric detection has been used to separate and quantitate ascorbic acid and dehydroascorbic acid. These components of vitamin C are resolved on a Lichrosorb-NH2column. The technique is capable of quantitatively following oxidation of ascorbic acid to dehydroascorbic acid and the reverse reduction. The technique is demonstrated to be suitable for assay of vitamin C in biological samples, foods, and pharmaceutical vitamin preparations.