Scale-up of human embryonic stem cell culture using a hollow fibre bioreactor
Scale-up of human embryonic stem cell culture using a hollow fibre bioreactor
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DOI：
10.1007/s10529-012-1033-1
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发表时间：
2012-12-01
影响因子：
2.7
通讯作者：
Mason, Chris
中科院分区：
文献类型：
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作者：
Roberts, Iwan;Baila, Stefano;Mason, Chris
人类胚胎干细胞衍生的细胞疗法用于大患者群体的商业化依赖于最小化昂贵和可变的手动处理方法，同时实现规模经济。量子细胞扩增系统是一种中空纤维生物反应器（Terumo BCT），用于试点研究，将6000万个人类胚胎干细胞扩增至7.08亿个细胞。通过在整个运行中优化培养基流速以更好地控制细胞微环境，可以预期进一步的改进。在生物反应器上维持高水平的多能性标志物表达，其中97.7%的细胞在收获时表达SSEA-4。
The commercialisation of human embryonic stem cell derived cell therapies for large patient populations is reliant on both minimising expensive and variable manual-handling methods whilst realising economies of scale. The Quantum Cell Expansion System, a hollow fibre bioreactor (Terumo BCT), was used in a pilot study to expand 60 million human embryonic stem cells to 708 million cells. Further improvements can be expected with optimisation of media flow rates throughout the run to better control the cellular microenvironment. High levels of pluripotency marker expression were maintained on the bioreactor, with 97.7 % of cells expressing SSEA-4 when harvested.