Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and non-ortho coplanar PCBs.
Evaluation of mitogen-induced responses in marine mammal and human lymphocytes by in-vitro exposure of butyltins and non-ortho coplanar PCBs.
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DOI：
10.1016/s0269-7491(02)00155-0
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发表时间：
2002-12
影响因子：
8.9
通讯作者：
H. Nakata;Akihito Sakakibara;M. Kanoh;S. Kudo;H. Watanabe;N. Nagai;N. Miyazaki;Y. Asano;S. Tanabe
中科院分区：
文献类型：
--
作者：
H. Nakata;Akihito Sakakibara;M. Kanoh;S. Kudo;H. Watanabe;N. Nagai;N. Miyazaki;Y. Asano;S. Tanabe
研究了丁基锡化合物(BTS：三丁基锡；TBT，二丁基锡；DBT和单丁基锡；MBT)和非邻位共面多氯联苯(IUPAC 77、126和169)对海洋哺乳动物和人类淋巴细胞的影响。从江豚(Phocoenoids Dalli)、宽吻海豚(Tursioptruncatus)、加州海豹(Zalophus Calfornianus)、大海豹(Phoca Largha)和人类(Homo Sapiens)分离的外周血单核细胞(PBMC)在不同浓度的BTS和共面多氯联苯中暴露。300 nM(ng/ml)的TBT和330 nM(77 ng/ml)的DBT对刀豆蛋白A(ConA)刺激的细胞有丝分裂有明显的抑制作用(P<；0.01)，而MBT在3600 nM(620 ng/ml)的处理水平下几乎没有细胞毒性。日本近海Dall‘s江豚肝脏中的BTS浓度在TBT为81-450 ng/g和200-1100 ng/g(湿重)之间。对于DBT，这超过了本研究中登记的细胞毒性水平。相反，非邻位共面的多氯联苯在浓度高达30 nM(10 ng/ml)时并不抑制细胞增殖。共面多氯联苯在江豚鲸脂中的残留量为0.12-1.3 ng/g，比细胞增殖的残留量低一个数量级。当细胞暴露在TBT/DBT和共面多氯联苯的混合物中时，细胞的增殖分别被显著降低到33 nM DBT+34 nM CB-77和33 nM DBT+28 nM CB-169混合物。关于海洋哺乳动物中污染物诱导的免疫抑制的研究主要集中在持久性有机氯化合物，如多氯联苯、杀虫剂和二恶英化合物。然而，这项研究表明，BTS的可能性也可能对自由放养的海洋哺乳动物和人类的免疫功能构成严重威胁。
The effects of exposure to butyltin compounds (BTs: tributyltin; TBT, dibutyltin; DBT and monobutyltin; MBT) and non-ortho coplanar PCBs (IUPAC 77, 126 and 169) on marine mammals and human lymphocyte were evaluated. Peripheral blood mononuclear cells (PBMCs) isolated from Dall's porpoises (Phocoenoides dalli), bottlenose dolphins (Tursiops truncatus), a California sealion (Zalophus californianus), a larga seal (Phoca largha) and humans (Homo sapiens) were exposed at varying concentrations of BTs and coplanar PCBs. Concanavalin A (Con A)-stimulated mitogenesis found significantly suppressed (P<0.01) when the cells were exposed at 300 nM (89 ng/ml) of TBT and 330 nM of DBT (77 ng/ml), while MBT showed little cytotoxicity at treatment levels of up to 3600 nM (620 ng/ml). BTs concentrations in the liver of Dall's porpoises from Japanese coastal waters ranged between 81–450 ng/g for TBT and 200–1100 ng/g (wet wt.) for DBTs, which is greater than the cytotoxic levels registered in this study. In contrast, non-ortho coplanar PCBs did not suppress cell proliferation at concentrations of up to 30 nM (10 ng/ml). The residue levels of coplanar PCBs in the blubber of Dall's porpoises were 0.12–1.3 ng/g, which were one order of lower than those levels that do cell proliferation. When cells were exposed to a mixture of TBT/DBTand coplanar PCBs, the proliferation was significantly reduced to 33 nM DBT plus 34 nM CB-77 and 33 nM DBT plus 28 nM CB-169 mixtures, respectively. The investigations relating the contaminant-induced immunosuppression in marine mammals have been focused on persistent organochlorines such as PCBs, pesticides and dioxin compounds. However, this study suggested the possibility of BTs could also pose a serious threat to the immune functions in free-ranging marine mammals and humans.