Improvement of the Calcitonin Radioimmunoassay
Improvement of the Calcitonin Radioimmunoassay
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降钙素放射免疫测定法的改进
DOI：
10.1515/cclm.1979.17.12.781
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发表时间：
1979
影响因子：
5.7
通讯作者：
P. Scriba
中科院分区：
文献类型：
--
作者：
W. G. Wood;O. Müller;G. Stalla;P. Scriba
本文介绍了一种快速、特异地测定人血清降钙素的方法。总检测时间在24 h以内，采用双抗体结合聚乙二醇分离结合和游离激素。通过使用Scatchard图检查抗体与人血清降钙素的亲和力。甲状腺髓样癌患者血清的几何稀释度与测量值呈线性关系，接近预期值。本文研究了用弗罗里硅土从血清中提取人血清降钙素，制备“无人降钙素”血清标准品的方法。对52例正常志愿者和99例甲状腺髓样癌或其他可能的降钙素代谢紊乱患者进行了检查。只有甲状腺髓样癌患者显示出显着升高的结果。在其他组中，2例嗜铬细胞瘤患者和1例29支气管癌患者血清降钙素水平升高。
A rapid and specific assay for human serum calcitonin is described. The total assay-time is under 24 h and uses double-antibody in combination with polyethylene glycol to separate bound and free hormone. The antibody has been checked for its affinity to human serum calcitonin by using a Scatchard plot. Geometric dilution of sera from patients with medullary thyroid carcinoma showed a linear relationship with measured values approximating those expected. The use of Florisil to extract human serum calcitonin from sera to make "human calcitonin-free"-serum for standards has been investigated. A series of 52 normal volunteers and 99 patients with medullary thyroid carcinoma or other possible calcitonin-metabolism disorders have been examined. Only the medullary thyroid carcinoma-patients showed significantly elevated results. In the other groups two patients with phaeochromocytoma and one patient from 29 with bronchial carcinoma had elevated serum calcitonin levels.