Surface enhanced Raman gene probe for HIV detection
Surface enhanced Raman gene probe for HIV detection
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DOI：
10.1021/ac970901z
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发表时间：
1998-04-01
影响因子：
7.4
通讯作者：
Vo-Dinh, T
中科院分区：
文献类型：
--
作者：
Isola, NR;Stokes, DL;Vo-Dinh, T
我们首次报道了使用表面增强拉曼 (SERS) 活性标记作为引物，用于聚合酶链式反应扩增特定目标 DNA 序列。该方法具有将 SERS 技术的光谱选择性和高灵敏度与 DNA 序列杂交提供的固有分子特异性相结合的潜力。使用人类免疫缺陷病毒的gag基因序列证明了检测方案的有效性。讨论了多个探针同时检测多个生物靶标的潜在用途。
We report, for the first time, the use of surface-enhanced Raman (SERS)-active labels for primers used in polymerase chain reaction amplification of specific target DNA sequences. This method has the potential for combining the spectral selectivity and high sensitivity of the SERS technique with the inherent molecular specificity offered by DNA sequence hybridization. The effectiveness of the detection scheme is demonstrated using the gag gene sequence of the human immunodeficiency virus. The potential use of multiple probes for simultaneous detection of multiple biological targets is discussed.