Restricted T cell receptor usage in DA rats during early collagen-induced arthritis.
Restricted T cell receptor usage in DA rats during early collagen-induced arthritis.
复制标题
早期胶原诱导的关节炎期间 DA 大鼠中 T 细胞受体的使用受到限制。
DOI：
10.1002/eji.1830240833
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发表时间：
1994
影响因子：
5.4
通讯作者：
Gold,DP
中科院分区：
文献类型：
--
作者：
Erlandsson,H;Mussener,A;Klareskog,L;Gold,DP
我们研究了T细胞受体（TcR）Vβ基因结构在经历同源胶原诱导关节炎的DA大鼠中的使用，该关节炎是通过用弗氏不完全佐剂乳化的同源II型胶原免疫诱导的。在半定量聚合酶链反应试验中分析了新鲜分离的炎症滑膜组织（ST）和原发引流淋巴结中的TcR Vβ表达。在临床疾病发作后2天的ST样品中观察到高度限制的TcR Vβ基因表达，其中Vβ6、8.2和8.5占所测量的总Vβ表达的60%以上。相应的主要引流淋巴结从患病的动物在这个时候表现出一定的Vβ表达的偏见，但不像在ST中发现的那样显著。发病后一周，一致的Vβ基因的偏见是不太明显的，在任何一个网站。这些结果表明，在疾病早期浸润滑膜的T细胞在Vβ表达方面受到高度限制。
We have investigated the usage of T cell receptor (TcR) Vβ gene structures in DA rats undergoing homologous collagen‐induced arthritis induced by immunization with homologous collagen type II emulsified in Freund's incomplete adjuvant. TcR Vβ expression within freshly isolated inflamed synovial tissue (ST) and primary draining lymph nodes was analyzed in a semi‐quantitative polymerase chain reaction assay. A highly restricted TcR Vβ gene expression was observed in ST samples 2 days after onset of clinical disease, with Vβ6, 8.2 and 8.5 comprising more than 60% of the total Vβ expression measured. The corresponding primary draining lymph nodes from the diseased animals showed some bias in Vβ expression at this time but not as remarkable as that found in ST. One week after onset of disease, consistent Vβ gene bias was much less evident in either site. These results indicate that T cells which infiltrate synovia early in disease are highly restricted with respect to Vβ expression.