Long non-coding RNA TUG1 recruits miR-29c-3p from its target gene RGS1 to promote proliferation and metastasis of melanoma cells
Long non-coding RNA TUG1 recruits miR-29c-3p from its target gene RGS1 to promote proliferation and metastasis of melanoma cells
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DOI：
10.3892/ijo.2019.4699
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发表时间：
2019-04-01
影响因子：
5.2
通讯作者：
Liu, Aimin
中科院分区：
文献类型：
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作者：
Wang, Yanqian;Liu, Gang;Liu, Aimin
黑色素瘤是一种侵袭性类型的皮肤癌，其特征在于全球范围内的高死亡率。因此，确定新的黑色素瘤诊断标志物和治疗靶点势在必行。越来越多的证据表明长链非编码RNA（lncRNA）在肿瘤的发生和发展中起着重要作用。近年来研究发现，牛磺酸上调的lncRNA 1（TUG 1）在肿瘤中的表达相对高于正常细胞，并促进多种肿瘤的发生发展。然而，TUG 1在黑色素瘤中的表达模式和作用机制仍不清楚。本研究的目的是研究TUG 1在黑色素瘤组织中的表达是否相对较高，以及这种表达是否与总生存率低相关。发现TUG 1的敲低抑制黑素瘤细胞的生长和转移并诱导细胞凋亡。相反，TUG 1的过表达促进黑色素瘤细胞的生长和转移，并抑制其凋亡。此外，本研究的结果表明，TUG 1隔离内源性miR-29 c-3 p，并能够抑制其表达。此外，观察到miR-29 c-3 p可以逆转TUG 1对黑色素瘤进展的促进作用，这可能与miR-29 c-3 p的靶基因G蛋白信号转导调节因子1（RGS 1）的正调控有关。综上所述，本研究的数据表明，TUG 1通过调节miR-29 c-3 p及其靶基因RGS 1促进黑色素瘤细胞的增殖和侵袭，并抑制细胞凋亡。因此，lncRNA TUG 1似乎是黑色素瘤患者的有希望的诊断标志物。
Melanoma is an aggressive type of skin cancer, characterized by high mortality rates worldwide. Therefore, the identification of new diagnostic markers and therapeutic targets for melanoma is imperative. Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play important roles in tumor initiation and progression. It was recently reported that the expression of lncRNA taurine upregulated 1 (TUG1) was relatively higher in cancer compared with that in normal cells, and that TUG1 promoted the progression of various cancers. However, the pattern of expression and mechanism of action of TUG1 in melanoma remain unclear. The aim of the present study was to investigate whether TUG1 expression is relatively higher in melanoma tissues and whether this expression is correlated with poor overall survival. Knockdown of TUG1 was found to suppress melanoma cell growth and metastasis and induce cell apoptosis. By contrast, the overexpression of TUG1 promoted the growth and metastasis of melanoma cells, and inhibited their apoptosis. In addition, the results of the present study indicated that TUG1 sequestered endogenous miR-29c-3p and that it was able to suppress its expression. Furthermore, it was observed that miR-29c-3p could reverse the promoting effect of TUG1 on melanoma progression, which may be associated with the positive regulation of regulator of G-protein signaling 1 (RGS1), a target gene of miR-29c-3p. Taken together, the data of the present study demonstrated that TUG1 promoted proliferation and invasion and suppressed apoptosis in melanoma cells by regulating miR-29c-3p and its target gene, RGS1. Therefore, lncRNA TUG1 appears to be a promising diagnostic marker for melanoma patients.