Early detection of invasive species in marine ecosystems using high-throughput sequencing: technical challenges and possible solutions
Early detection of invasive species in marine ecosystems using high-throughput sequencing: technical challenges and possible solutions
复制标题
使用高通量测序早期检测海洋生态系统中的入侵物种：技术挑战和可能的解决方案
DOI：
10.1007/s00227-016-2911-1
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发表时间：
2016-05
期刊：
影响因子：
2.4
通讯作者：
Aibin Zhan
中科院分区：
文献类型：
--
作者：
Wei Xiong;Haitao Li;Aibin Zhan
引进和传播入侵的非土著物种显然是海洋和沿海生态系统中一个日益严重的全球性问题。早期发现NIS已被认为是管理计划的首要任务和有效方法之一。然而，基于传统形态方法的一系列稀有NIS的检测在海洋领域面临着巨大的技术挑战，因为许多研究较少的生物可能很小和/或没有足够的形态特征。基于DNA的方法的使用大大提高了检测的准确性和效率。高通量测序技术(HTS)具有检测极低丰度物种的能力强、单位测序成本低等优点，已被广泛应用于海洋NIS的早期检测。尽管基于HTS的方法已被批准用于水生生态系统中NIS的可靠普查，特别是对于那些存在极低丰度的NIS，但实验设计和数据收集中的许多因素都会导致错误，包括假阴性和假阳性。在这里，我们确定了导致这两种错误类型的因素，讨论了原因和后果，并提出了利用HTS早期检测海洋和沿海生态系统中的NIS的可能解决方案。
Introduction and spread of invasive non-indigenous species (NIS) has been clearly recognized as a growing global problem in marine and coastal ecosystems. Early detection of NIS has been recognized as one of the first priorities and effective ways in management programs. However, the detection of an array of rare NIS based on traditional morphological methods represents enormous technical challenges in the marine realm, as numerous poorly studied organisms may be small and/or have insufficient morphological distinctive features. The use of DNA-based approaches has largely increased the detection accuracy and efficiency. Owing to many obvious advantages such as the high capacity to detect extremely low-abundance species and low cost per sequence, high-throughput sequencing (HTS)-based methods have been popularly proposed for early detection of marine NIS. Although HTS-based approaches have been approved robust for the census of NIS in aquatic ecosystems, especially for those present at extremely low abundance, many factors in experimental design and data collection can cause errors including both false negatives and false positives. Here we identify factors responsible for both error types, discuss causes and consequences, and propose possible solutions when utilizing HTS for early detection of NIS in marine and coastal ecosystems.
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影响因子：
9.2
作者：
Zhou X;Li Y;Liu S;Yang Q;Su X;Zhou L;Tang M;Fu R;Li J;Huang Q
通讯作者：
Huang Q
DOI：
10.1111/j.1471-8286.2007.01678.x
发表时间：
2007-05-01
期刊：
Molecular ecology notes
影响因子：
--
作者：
Ratnasingham S;Hebert PD
通讯作者：
Hebert PD
影响因子：
2.6
作者：
Flynn, Jullien M.;Brown, Emily A.;Chain, Frederic J. J.;MacIsaac, Hugh J.;Cristescu, Melania E.
通讯作者：
Cristescu, Melania E.
DOI：
--
发表时间：
2004
期刊：
--
影响因子：
--
作者：
W. Thompson
通讯作者：
W. Thompson
影响因子：
1.2
作者：
M. Dawood
通讯作者：
M. Dawood