Elsinoe australis Causing Spot Anthracnose on Poplar in China
Elsinoe australis Causing Spot Anthracnose on Poplar in China
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澳大利亚Elsinoe australis引起我国杨树斑炭疽病
DOI：
10.1094/pdis-11-19-2349-re
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发表时间：
2020-08-01
期刊：
影响因子：
4.5
通讯作者：
Cheng, Qiang
中科院分区：
文献类型：
--
作者：
Zhao, Lijuan;Xiao, Hongju;Cheng, Qiang
白杨人工林是我国重要的工业原料林。在中国东南部的毛白杨和美洲黑杨叶片上观察到红斑症状。根据形态学和分子系统发育分析，从病斑上分离的真菌被鉴定为南方痂囊腔菌（Elsinoe australis），该菌以前被认为是柑橘属的病原菌。和荷荷芭，但在中国尚未报道。致病性试验发现，从两个白杨品种的分离物引起的红斑症状，从不同的白杨品种的叶片上，也导致结痂的果实上的一个杂交柑橘，但不是对水果的橙子，柠檬，或葡萄柚。一株E. australis分离物。对大肠杆菌MAT 1 -1和MAT 1 -2基因座的遗传结构进行了研究。australis的基因组序列和长距离PCR分析结果表明，个体特异性PCR证实，单个菌株只携带两种相反交配型之一，表明异宗配合系统。我们的结果不仅揭示了一个新的E. australis致病型的研究，并提供了其基因组和交配系统信息。
Poplar plantations provide important industrial feedstock in China. Red spot symptoms were observed on leaves of Populus tomentosa and P. deltoides in southeastern China. Based on morphology and molecular phylogenetic analysis, the fungus isolated from disease spots was identified as Elsinoe australis, which has been previously recognized as a pathogen of Citrus spp. and jojoba but has not been reported in China. Pathogenicity tests found that isolates from two poplar species caused red spot symptoms on leaves from different poplar species and also led to scab formation on the fruit of one hybrid citrus but not on fruit of orange, lemon, or grapefruit. The draft genome of one E. australis isolate was generated. The genetic architecture of the MAT1-1 and MAT1-2 loci of E. australis was revealed by genome sequence and long-range PCR analyses. Single isolates carried only one of two opposite mating-types was confirmed by idiomorph-specific PCR, suggesting a heterothallic mating system. Our results not only revealed a new E. australis pathotype causing poplar spot anthracnose in China but also provided its genome and mating system information.