Atypical patterns of neural infection produced in mice by drug-resistant strains of herpes simplex virus.
Atypical patterns of neural infection produced in mice by drug-resistant strains of herpes simplex virus.
复制标题
单纯疱疹病毒耐药株在小鼠体内产生的非典型神经感染模式。
作者：
H. Field;J. Anderson;P. Wildy
脑内（i.c.）在接种HSV突变株后1至2个月发现发生白内障。白内障的形成随后被证明是在接种后1周内开始的急性视网膜炎之后。该突变体已被选择为对核苷类似物阿昔洛韦具有高抗性，并且先前已被证明在胸苷激酶的诱导中存在缺陷，并且还表达改变的DNA聚合酶。i.c.大于10（5）p.f.u.。相比之下，约7 p.f.u.对于亲本菌株。i.c.后病毒复制的研究用亚致死剂量的突变体接种显示，在脑中仅产生少量感染性病毒，但在接种后6 - 12天期间，在视网膜组织中发生剧烈复制，产生非常高滴度的病毒。
Mice inoculated intracerebrally (i.c.) with a mutant strain of HSV were found to develop cataracts 1 to 2 months after inoculation. Cataract formation was subsequently shown to follow an acute retinitis which commenced within 1 week of inoculation. The mutant had been selected for high resistance to the nucleoside analogue acyclovir and has been shown previously to be defective in the induction of thymidine kinase and also to express an altered DNA polymerase. The LD50 for mice inoculated i.c. was greater than 10(5) p.f.u. compared with approx 7 p.f.u. for the parental strain. Studies of virus replication following i.c. inoculation with a sublethal dose of the mutant revealed that only small amounts of infectious virus were produced in the brain, but during a period from 6 to 12 days after inoculation vigorous replication occurred in retinal tissue, producing very high titres of virus.