Diet-induced obesity and hepatic gene expression alterations in C57BL/6J and ICAM-1-deficient mice
Diet-induced obesity and hepatic gene expression alterations in C57BL/6J and ICAM-1-deficient mice
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DOI：
10.1152/ajpendo.00072.2001
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发表时间：
2002-03-01
影响因子：
5.1
通讯作者：
West, DB
中科院分区：
文献类型：
--
作者：
Gregoire, FM;Zhang, Q;West, DB
在细胞间粘附分子-1（ICAM-1）基因敲除的C57 BL/6 J（B6）雄性小鼠中，评价了高脂喂养对肥胖发展的影响。在基线和饮食1、11和50天后收集血清和组织。在饮食11天后，ICAM-1缺乏，但不是B6，小鼠发展为脂肪肝，并显示腹股沟脂肪垫重量显着增加。在第50天，ICAM-1缺陷小鼠体重减轻，其肥胖指数和循环瘦素水平显着低于B6对照组。为了更好地理解对饮食的早期差异反应，通过使用Affyssin基因芯片在三个时间点分析肝脏基因表达。在这两种菌株中，在对高脂肪饮食的反应中检测到类似的基因表达模式。然而，固醇调节元件结合蛋白-1，载脂蛋白A4，和adipsin的mRNA显着诱导ICAM-1缺陷的肝脏，这表明这些基因及其相关的途径可能参与在敲除小鼠中观察到的急性饮食反应。
The effects of high-fat feeding on the development of obesity were evaluated in intercellular adhesion molecule-1 (ICAM-1) knockout and C57BL/6J (B6) male mice fed a high-fat diet for less than or equal to50 days. Serum and tissues were collected at baseline and after 1, 11, and 50 days on the diet. After 11 days on the diet, ICAM-1-deficient, but not B6, mice developed fatty livers and showed a significant increase in inguinal fat pad weight. At day 50, ICAM-1-deficient mice weighed less, and their adiposity index and circulating leptin levels were significantly lower than those of B6 controls. To better understand the early differential response to the diet, liver gene expression was analyzed at three time points by use of Affymetrix GeneChips. In both strains, a similar pattern of gene expression was detected in response to the high-fat diet. However, sterol regulatory element-binding protein-1, apolipoprotein A4, and adipsin mRNAs were significantly induced in ICAM-1-deficient livers, suggesting that these genes and their associated pathways may be involved in the acute diet response observed in the knockout mice.