Effect of quorum sensing inducers and inhibitors on male-killing Wolbachia, the endosymbiont of the adzuki bean borer, Ostrinia scapulalis (Lepidoptera: Crambidae)
Effect of quorum sensing inducers and inhibitors on male-killing Wolbachia, the endosymbiont of the adzuki bean borer, Ostrinia scapulalis (Lepidoptera: Crambidae)
复制标题
DOI：
10.1007/s13355-023-00825-w
复制
发表时间：
2023-04-15
影响因子：
1.3
通讯作者：
Tagami，Yohsuke
中科院分区：
文献类型：
--
作者：
Hidayanti，Ardhiani Kurnia;Sugimoto，Takafumi N.;Tagami，Yohsuke
红小豆螟Ostrinia scapulalis（步行者）（鳞翅目：草螟科）是红小豆及其它双子叶植物的重要害虫。scapulalis在昆虫宿主的胚胎和幼虫阶段导致雄性死亡。先前的研究报道了Wolbachiadensity动态和后代的性别表型之间的关联。在这项研究中，我们处理了O.用3 O-C12-HSL作为群体感应诱导剂和4-苯基丁醇作为群体感应抑制剂，在成虫和幼虫阶段进行肩形幼虫实验。QS诱导剂处理后，Wolbachia密度与雌虫比例呈显著正相关，而QS抑制剂处理后Wolbachia密度与雌虫比例呈显著正相关。QS诱导剂和抑制剂处理能稳定O.肩胛肌。
Ostrinia scapulalis(Walker) (Lepidoptera: Crambidae), the adzuki bean borer, is a serious pest in adzuki bean plants and other dicotyledons.Wolbachia, the insect symbiont that infectsO. scapulalis, causes male-killing during the embryonic and larval stages in the insect host. Previous studies have reported an association betweenWolbachiadensity dynamics and the sex phenotype of offspring. In this study, we treatedO. scapulalisat the adult and larval stages using 3O-C12-HSL as a quorum sensing (QS) inducer and 4-phenylbutanol as a QS inhibitor. QS inducer treatment in the adult stage increased the density ofWolbachiainO.scapulalis, and there was a strong positive correlation betweenWolbachiadensity and female proportion in the adult stage after QS inhibitor treatment. QS inducer and inhibitor treatments in the larval stage stabilizeWolbachiadensity inO. scapulalis.