Effects of clofibrate and acetylsalicylic acid on hepatic carnitine palmitoyltransferase synthesis.
Effects of clofibrate and acetylsalicylic acid on hepatic carnitine palmitoyltransferase synthesis.
复制标题
安妥明和乙酰水杨酸对肝肉毒碱棕榈酰转移酶合成的影响。
DOI：
10.1016/0006-2952(89)90235-9
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发表时间：
1989
影响因子：
5.8
通讯作者：
Brady,LJ
中科院分区：
文献类型：
--
作者：
Brady,PS;Brady,LJ
氯贝特和乙酰水杨酸都被报道在给药时增加肉碱棕榈酰基转移酶(CPT)的活性。本研究的目的是确定CPT活性增加的机制。大鼠体重150~200g，分别饲喂饲料、添加0.5%氯贝特的饲料或添加1%乙酰水杨酸的饲料2周。在这段时间结束时，氯贝特组和乙酰水杨酸组的肝脏CPT活性分别是对照组的4倍和3.6倍。免疫反应蛋白分别比对照组增加了4.0倍和3.6倍。氯贝特组和乙酰水杨酸组大鼠肝细胞核转录速率分别为对照组的2.8倍和1.9倍，肝组织的mRNA水平分别为对照组的2.8倍和2.0倍。这些数据表明，给予氯贝特和乙酰水杨酸引起的CPT活性的增加，至少部分是由于CPT蛋白的增加，导致CPT特异的转录速率和mRNA水平的增加。
Clofibrate and acetylsalicylic and have both been reported to increase carnitine palmitoyltransferase (CPT) activity when administered to rats. The purpose of the present study was to determine the mechanism of the increase in CPT activity. Rats (150–200 g) were fed one of the following: chow, chow with 0.5% clofibrate, or chow with 1% acetylsalicylic acid for 2 weeks. At the end of this time, hepatic CPT activity was increased 4-fold over control in the clofibrate group and 3.6-fold over control in the acetylsalicylic acid group. Immunoreactive protein increased 4.0- and 3.6-fold, respectively, over control. Transcription rates of hepatic nuclei were increased 2.8- and 1.9-fold over control in the clofibrate and acetylsalicylic add groups, and hepatic mRNA levels increased 2.8- and 2.0-fold respectively. These data indicate that increases in CPT activity caused by clofibrate and acetylsalicylic acid administration are due, at least in part, to increased CPT protein, resulting from increased transcription rate and levels of mRNA specific for CPT.