MeCP2 and MBD2 expression in human neoplastic and non-neoplastic breast tissue and its association with oestrogen receptor status.
MeCP2 and MBD2 expression in human neoplastic and non-neoplastic breast tissue and its association with oestrogen receptor status.
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DOI：
10.1038/sj.bjc.6601392
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发表时间：
2003-11-17
影响因子：
8.8
通讯作者：
Widschwendter, M
中科院分区：
文献类型：
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作者：
Muller, H M;Fiegl, H;Goebel, G;Hubalek, M M;Widschwendter, A;Muller-Holzner, E;Marth, C;Widschwendter, M
本研究使用定量实时PCR方法分析了人类非肿瘤性（n = 11）和肿瘤性（n = 57）乳腺组织标本中甲基CpG结合蛋白家族的两个成员MeCP 2和MBD 2的mRNA表达。我们观察到肿瘤组织中MeCP 2 mRNA的表达水平高于非肿瘤组织（P = 0.001），而MBD 2的表达水平无显著差异。当研究乳腺癌最重要的临床病理特征和两种MBD的mRNA表达水平之间的关系时，我们发现雌激素受体（OR）阳性乳腺癌标本中MeCP2 mRNA的水平高于OR阴性乳腺癌（P = 0.005）。此外，我们观察到，与表达低水平的那些相比，在表达高水平OR的非肿瘤组织中MeCP2的水平在统计学上显著更高（P = 0.017）。最后，使用线性回归模型，我们确定了OR表达和MeCP2 mRNA表达在肿瘤和非肿瘤乳腺组织标本中的统计学显著相关性（P = 0.003）。总之，我们能够首次证明OR状态与MeCP2 mRNA表达之间存在强相关性。此外，我们推测，MeCP2，调节OR，在人类乳腺组织的分化过程中发挥了关键作用。
This study analysed mRNA expression of two members of the methyl-CpG-binding protein family – MeCP2 and MBD2 – in human non-neoplastic (n=11) and neoplastic (n=57) breast tissue specimens using a quantitative real-time PCR method. We observed higher expression levels of MeCP2 mRNA in neoplastic tissues than in non-neoplastic tissues (P=0.001), whereas no significant differences for MBD2 were detected. When studying the relations between the most important clinicopathologic features of breast cancer and the mRNA expression level of both MBDs, we found that oestrogen receptor (OR)-positive breast cancer specimens contained higher levels of MeCP2 mRNA than did OR-negative cancers (P=0.005). Furthermore, we observed statistically significantly higher levels of MeCP2 in non-neoplastic tissues expressing high levels of OR as compared to those expressing low levels (P=0.017). Finally, using a linear regression model, we identified a statistically significant association between OR expression and MeCP2 mRNA expression in neoplastic and non-neoplastic breast tissue specimens (P=0.003). In conclusion, we were able to demonstrate for the first time that there exists a strong association between OR status and MeCP2 mRNA expression. Furthermore, we speculate that MeCP2, regulated by OR, plays a key role in the differentiation processes in human breast tissues.