SIMULTANEOUS PRESENCE OF DIFFERENT BORRELIA-BURGDORFERI GENOSPECIES IN BIOLOGICAL-FLUIDS OF LYME-DISEASE PATIENTS
SIMULTANEOUS PRESENCE OF DIFFERENT BORRELIA-BURGDORFERI GENOSPECIES IN BIOLOGICAL-FLUIDS OF LYME-DISEASE PATIENTS
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DOI：
10.1128/jcm.33.3.602-608.1995
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发表时间：
1995-03-01
影响因子：
9.4
通讯作者：
GODFROID, E
中科院分区：
文献类型：
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作者：
DEMAERSCHALCK, I;BENMESSAOUD, A;GODFROID, E
基于伯氏疏螺旋体 ospA 基因序列的寡核苷酸引物已设计用于 PCR 中，以对莱姆病涉及的伯氏疏螺旋体所有基因种（SL 引物）或每个基因种（GI 至 GIII 引物）进行分型。然后将这些基因种特异性引物用于对从 18 名神经疏螺旋体病患者收集的 24 份生物体液进行 PCR。在测试的样本中，20 个含有加氏疏螺旋体 DNA，11 个含有严格伯氏疏螺旋体 DNA，10 个含有阿氏疏螺旋体 DNA。总共有 10 名患者似乎感染了单一基因种，8 名患者感染了不止一种莱姆病相关基因种。六名患者的血清标本被异源抗原吸收并通过蛋白质印迹（免疫印迹）进行测试。在 4 例中，残留免疫检测揭示了 PCR 检测到的基因种的特定表位；其中两例的一致结果表明患者存在多重感染。在另外两例中，蛋白质印迹显示了两种疏螺旋体基因种的特异性抗体，而 PCR 只检测到了一种疏螺旋体基因种的 DNA。总之，数据强调了莱姆病多重感染的患病率相对较高，并证实了加氏疏螺旋体与神经疏螺旋体病的关联。
Oligonucleotide primers based on Borrelia burgdofero sensu late ospA gene sequences have been designed for use in the PCR to type all (SL primers) or each (GI to GIII primers) of the B. burgdoferi sensu late genospecies involved in Lyme disease. These genospecies-specific primers were then used in the PCR on 24 biological fluids collected from 18 neuroborreliosis patients. Among the samples tested, 20 contained DNA from Borrelia garinii, 11 contained DNA from B. burgdorferi sensu stricto, and 10 contained DNA from Borrelia afzelii. In toto, 10 patients appeared to have been infected by a single genospecies and 8 were infected by more than one Lyme disease-associated genospecies. Serum specimens from six patients were absorbed with heterologous antigens and tested by Western blotting (immunoblotting). In four cases, residual immunodetection revealed specific epitopes of genospecies also detected by PCR; in two of them, the concordant results indicated pluri-infection of the patients. In the other two cases, Western blotting showed specific antibodies for two genospecies of Borrelia, while PCR detected DNA from only one. In summary, the data underscored the relatively high prevalence of pluri-infections in Lyme disease and confirmed the association of B. garinii with neuroborreliosis.