Potential role of Noxes in the protection of mucosae: H2O2 as a bacterial repellent
Potential role of Noxes in the protection of mucosae: H2O2 as a bacterial repellent
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DOI：
10.1016/j.micinf.2009.02.009
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发表时间：
2009-04-01
影响因子：
5.8
通讯作者：
Allaoui, A.
中科院分区：
文献类型：
--
作者：
Botteaux, A.;Hoste, C.;Allaoui, A.
Duox蛋白是NADPH氧化酶（Nox）家族的成员，负责各种组织类型（包括支气管和肠粘膜）产生过氧化氢（H2 O2）。H2 O2对白细胞和巨噬细胞的抗菌杀伤作用通常被认为是其功能的范例。我们在这里调查的积极作用H2 O2在预防沙门氏菌的细胞入侵。我们表明，过氧化氢，在保存细菌生长的条件下，具有对沙门氏菌运动琼脂平板上的驱避作用。此外，大鼠甲状腺细胞系PCCl 3产生的H2 O2可使细胞的细菌入侵减少约40%。为了测试观察到的表型是否归因于H2 O2产生，我们构建了在细胞表面表达Duox 2蛋白的CHO稳定细胞系（CHO-D2）。转染的细胞产生大量的H2 O2。在感染沙门氏菌后，CHO-D2细胞的侵袭减少了高达60%。在表达PCCl 3和CHO的Duox 2细胞中，与过氧化氢酶孵育后恢复正常侵袭。我们的数据表明，降低浓度的H2 O2可以作为细菌的驱避剂，使它们远离细胞，这种情况可以自然地防止体内粘膜细胞感染。(C)2009年Elsevier Masson SAS。All rights reserved.
Duox proteins are members of the NADPH oxidase (Nox) family and are responsible for hydrogen peroxide (H2O2) production by various tissue types including bronchial and intestinal mucosae. The antimicrobial killing role of H2O2 in leukocytes and macrophages is generally considered as the paradigm of its function. We investigated here the positive role of H2O2 in the prevention of cellular invasion by Salmonella. We show that H2O2, under conditions that preserved bacterial growth, has a repellent effect on Salmonella motility on agar plates. In addition, H2O2 produced by PCCl3, a rat thyroid cell line, reduces bacterial invasion of the cells by around 40%. To test whether the observed phenotype is attributable to H2O2 production, we constructed a CHO stable cell line expressing Duox2 protein at the cell surface (CHO-D2). The transfected cells produce a high amount of H2O2. Upon infection with Salmonella, the invasion of CHO-D2 cells was reduced by up to 60%. In both PCCl3 and CHO expressing Duox2 cells, normal invasion was restored upon incubation with catalase. Our data suggest that H2O2 at reduced concentrations acts as a repellent for bacteria, keeping them away from cells, a situation that could naturally prevent mucosal cells infection in vivo. (C) 2009 Elsevier Masson SAS. All rights reserved.