In-depth Proteomic mapping of mouse (Mus musculus) epididymal constructive basis for sperm maturation.
In-depth Proteomic mapping of mouse (Mus musculus) epididymal constructive basis for sperm maturation.
复制标题
小鼠（小家鼠）附睾精子成熟构建基础的深入蛋白质组学图谱。
DOI：
10.1186/s12953-015-0076-3
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发表时间：
2015
期刊：
影响因子：
2
通讯作者：
Wang YW
中科院分区：
文献类型：
--
作者：
Liu X;Liu FJ;Jin SH;Shen XF;Wang YW
小鼠附睾在精子成熟过程中起着重要作用，但目前仍缺乏关于小鼠附睾蛋白表达的全面数据。本研究首次对小鼠附睾蛋白质组进行了深入的凝胶电泳分析，并建立了双向电泳图谱。共检测到832个蛋白质点的重复性凝胶，625点对应的355个独特的蛋白质条目已成功地确定了MALDI-TOF-MS。蛋白质组数据的置信度进行了验证，通过Western blot。功能注释表明，这些蛋白质主要与一般代谢、抗氧化和结构分子活性有关。免疫组化显示两种结构蛋白（肌球蛋白调节轻多肽9和α-2型I型胶原）自产后持续表达于肌样细胞。本研究提供了小鼠附睾蛋白质组的初步参考图谱，这将极大地扩展附睾结构基础的知识，有助于更好地了解小鼠附睾精子成熟过程中的蛋白质。本文的在线版本（doi：10.1186/s12953-015-0076-3）包含补充材料，可供授权用户使用。
The mouse epididymis performs an essential role in sperm maturation, but global protein expression data in mouse epididymis are still lacking. Here, we reported the first in-depth gel-based profiling of mouse epididymis proteome and established a 2-DE map. A total of 832 protein spots were detected in the reproducible gels, and 625 spots corresponding to 355 unique protein entries have been successfully identified by MALDI-TOF-MS. The confidence of proteome data was validated by Western blot. Functional annotations showed that these proteins were mainly related to general metabolism, antioxidant and structural molecule activity. Immunohistochemistry disclosed two structural proteins (myosin regulatory light polypeptide 9 and alpha-2 type I collagen) continuously expressed in the myoid cell since postpartum. This study provides a first-draft reference map of the mouse epididymis proteome, which will greatly expand the knowledge of the epididymal structural basis and contribute to the better understanding of those proteins in the process of mouse epididymal sperm maturation. The online version of this article (doi:10.1186/s12953-015-0076-3) contains supplementary material, which is available to authorized users.