Using a large area CMOS APS for direct chemiluminescence detection in Western blotting electrophoresis
Using a large area CMOS APS for direct chemiluminescence detection in Western blotting electrophoresis
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使用大面积 CMOS APS 进行蛋白质印迹电泳中的直接化学发光检测
DOI：
10.1117/12.911615
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Esposito M
中科院分区：
文献类型：
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作者：
Esposito M
Western印迹电泳测序是一种广泛用于功能蛋白质组学的分析技术，用于检测、识别和定量生物样品中的特异性标记蛋白。用于蛋白质印迹的常用标记物是基于鲁米诺在425 nm处的荧光发射的增强化学发光（ECL）试剂。胶片乳剂是常规的检测介质，但其特征在于非线性响应和有限的动态范围。已经开发了几种蛋白质印迹数字成像系统，主要基于使用解决这些问题的冷却电荷耦合器件（CCD）和单个雪崩二极管。即便如此，这些系统也存在关键的缺点，例如低帧速率和需要在低温下操作。使用互补金属氧化物半导体（CMOS）有源像素传感器（APS）的直接光学检测可以代表该应用的合适的数字替代方案。在本文中，作者证明了直接荧光检测的可行性，在蛋白质印迹电泳使用CMOS APS在室温下。此外，近年来，制造技术的改进已经使得用于非常大的成像器的可靠工艺可用，其现在可以按比例放大到晶片尺寸，从而允许全尺寸蛋白质印迹样品的直接接触成像。我们建议使用一种新型的晶圆级APS（12.8 cm×13.2 cm），其阵列架构使用两种不同的像素几何形状，可以同时提供固有的低噪声和高动态范围图像，这与当前的蛋白质印迹成像系统相比有了显着的改进。
Western blotting electrophoretic sequencing is an analytical technique widely used in Functional Proteomics to detect, recognize and quantify specific labelled proteins in biological samples. A commonly used label for western blotting is Enhanced ChemiLuminescence (ECL) reagents based on fluorescent light emission of Luminol at 425nm. Film emulsion is the conventional detection medium, but is characterized by non-linear response and limited dynamic range. Several western blotting digital imaging systems have being developed, mainly based on the use of cooled Charge Coupled Devices (CCDs) and single avalanche diodes that address these issues. Even so these systems present key drawbacks, such as a low frame rate and require operation at low temperature. Direct optical detection using Complementary Metal Oxide Semiconductor (CMOS) Active Pixel Sensors (APS)could represent a suitable digital alternative for this application. In this paper the authors demonstrate the viability of direct chemiluminescent light detection in western blotting electrophoresis using a CMOS APS at room temperature. Furthermore, in recent years, improvements in fabrication techniques have made available reliable processes for very large imagers, which can be now scaled up to wafer size, allowing direct contact imaging of full size western blotting samples. We propose using a novel wafer scale APS (12.8 cm×13.2 cm), with an array architecture using two different pixel geometries that can deliver an inherently low noise and high dynamic range image at the same time representing a dramatic improvement with respect to the current western blotting imaging systems.
用于化学发光检测的室温 CMOS 单光子传感器
DOI：
--
发表时间：
2006
期刊：
μ-TAS 2006 Vol 1
影响因子：
--
作者：
Marek Gersbach;Yuki Maruyama;Cristiano Niclass;Kazuaki Sawada;Edoardo Charbon
通讯作者：
Edoardo Charbon
基于 CCD 的系统，用于通过紫外吸收检测电泳凝胶中的 DNA。
影响因子：
3.5
作者：
Alex R Mahon;Alex R Mahon;John H MacDonald;Robert J. Ott;A. Mainwood
通讯作者：
A. Mainwood