The effect of influenza virus A on th1/th2 balance and alveolar fluid clearance in pregnant rats
The effect of influenza virus A on th1/th2 balance and alveolar fluid clearance in pregnant rats
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DOI：
10.3109/01902148.2011.587136
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发表时间：
2011-09-01
影响因子：
1.7
通讯作者：
Sakuma, Tsutomu
中科院分区：
文献类型：
--
作者：
Gu, Xiu;Li, Peng;Sakuma, Tsutomu
孕妇更容易感染H1N1病毒，并经常出现严重的并发症。研究了H1N1感染对孕鼠辅助性T细胞1/2型（Th 1/Th 2）平衡和肺泡液体清除率（AFC）的影响。孕鼠鼻内感染流感病毒。采用酶联免疫吸附试验（ELISA）检测外周血干扰素-γ（IFN-γ）和白细胞介素-4（IL-4），并通过肺泡灌洗液中白蛋白浓度估计AFC。在非妊娠大鼠中IFN-γ/IL-4的比率为21 +/- 7。感染妊娠大鼠的IFN-γ/IL-4比值（8 ± 3）显著低于正常妊娠大鼠（15 ± 8）。正常非妊娠大鼠的AFC为17% +/- 3%，H1N1感染将其降低至11% +/-2%。正常妊娠大鼠的AFC为22% +/- 2%，H1N1感染使其降至10% +/-2%。地塞米松在非妊娠组和妊娠组均逆转AFC（分别为14% +/- 4%和13% +/-2%）。这些结果表明，流感病毒A感染导致Th 2偏向的免疫，并降低AFC在正常大鼠，并进一步在妊娠大鼠这些。地塞米松在妊娠和非妊娠大鼠中逆转这些作用。
Pregnant women are more prone to H1N1 infection and often with severe complications. The authors studied the influence of H1N1 infection on T-helper cell type 1/type 2 (Th1/Th2) balance and alveolar fluid clearance (AFC) in pregnant rats. The pregnant rats were infected intranasally with influenza virus. Peripheral blood interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) were measured by enzyme-linked immunosorbent assay (ELISA) and AFC was estimated by albumin concentration in alveolar lavage. The ratio of IFN-gamma/IL-4 in nonpregnant rats was 21 +/- 7. There was significant increase in both cytokines in infected pregnant rats compared with noninfected counterparts, with dramatic reduction in IFN-gamma/IL-4 ratio (8 +/- 3) compared to that (15 +/- 8) in normal pregnant group. AFC of normal nonpregnant rats was 17% +/- 3% and H1N1 infection reduced it to 11% +/- 2%. AFC of normal pregnant rats was 22% +/- 2% and H1N1 infection reduced it to 10% +/- 2%. Dexamethasone reversed AFC in both nonpregnant and pregnant groups (14% +/- 4% and 13% +/- 2%, respectively). These results show that influenza virus A infection leads to Th2-biased immunity and reduces AFC in normal rats, and further worsens these in pregnant rats. Dexamethasone reverses these effects in both pregnant and nonpregnant rats.