MUC1/episialin: a critical barrier in the female reproductive tract
MUC1/episialin: a critical barrier in the female reproductive tract
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DOI：
10.1016/s0165-0378(99)00046-7
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发表时间：
1999-12-01
影响因子：
3.4
通讯作者：
Carson, DD
中科院分区：
文献类型：
--
作者：
DeSouza, MM;Surveyor, GA;Carson, DD
女性生殖道必须抵抗微生物感染，并支持胚胎发育，植入和胎盘形成。一般而言，生殖道粘蛋白，特别是Muc 1/episialin，在着床相关事件和防止微生物感染中起关键作用。下生殖道中高水平的粘蛋白表达可能提供针对感染的保护，而子宫粘蛋白的下调已被认为提供进入子宫表面的途径。目前的研究表明，粘蛋白，特别是Mud，是胚胎附着的有效屏障。此外，正常饲养的雌性Mud null小鼠的品系显示出下生殖道的慢性感染和炎症，生育率显著降低。当泥空菌被安置在无病原体的环境中时，没有观察到这种表型，表明这种表型是由慢性微生物暴露引起的。从受影响的Mud null小鼠的生殖道中仅分离出正常的内源性植物群，表明这些细菌物种随着粘蛋白屏障的丧失而变得机会性。葡萄球菌粘附下生殖道上皮细胞被认为是介导的细胞表面粘蛋白碳水化合物。总的来说，这些研究证明了泥浆在女性生殖道生理学的各个方面的关键屏障作用。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
The female reproductive tract must resist microbial infections as well as support embryonic development, implantation and placentation. Reproductive tract mucins, in general, and Muc1/episialin, in particular, play key roles in implantation related events and in protection from microbial infection. High levels of mucin expression in the lower reproductive tract presumably affords protection against infection while down-regulation of uterine mucins has been suggested to provide access to the uterine surface. The present studies demonstrate that mucins, particularly Mud, are effective barriers to embryo attachment. Furthermore, a strain of female Mud null mice in normal housing displays chronic infection and inflammation of the lower reproductive tract and markedly reduced fertility rates. This phenotype is not observed when Mud nulls are housed in a pathogen-free environment indicating that this phenotype results from chronic microbial exposure. Only normal endogenous flora were isolated from the reproductive tracts of affected Mud null mice, suggesting that these bacterial species become opportunistic with loss of the mucin barrier. Staphylococcal adherence to lower reproductive tract epithelia was found to be mediated by cell surface mucin carbohydrates. Collectively, these studies demonstrate a critical barrier role for Mud in various aspects of female reproductive tract physiology. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.