Tentative identification of key factors determining the hemostatic efficiency of diatom frustule
Tentative identification of key factors determining the hemostatic efficiency of diatom frustule
复制标题
初步确定决定硅藻壳止血效果的关键因素
DOI：
10.1039/d0bm02002h
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发表时间：
2021
影响因子：
6.6
通讯作者：
Han Jichang
中科院分区：
文献类型：
--
作者：
Wang Lulu;Pan Kehou;Zhang Lin;Zhou Chengxu;Li Yun;Zhu Baohua;Han Jichang
开发优良的快速止血材料变得越来越重要。我们之前的研究表明，锥形等中心硅藻在止血方面优于QuikClot®，但对羽状硅藻的相关研究仍然很少。羽状硅藻的形态和物理化学性质与中心硅藻有很大的不同，这意味着从止血效果上也可能观察到显著的差异。因此，本研究以四种羽状硅藻为研究对象，对其止血效果进行了研究。其中，所有的硅藻都表现出了出色的止血性能。例如，松材线虫的体外凝血时间(100.33±9.5S)比QuikClot®缩短了32.4%。大鼠断尾模型和股动脉模型的止血时间分别为82 S和180 S，仅为QuikClot®的1/2和1/3左右。此外，大鼠断尾和股动脉模型的出血量分别比QuikClot®减少73.4%和61%。此外，硅藻试管还表现出良好的生物相容性(溶血率<5%，MEFS细胞存活率<80%，无炎症反应)。为了找出锥体止血作用的关键因素，本研究进一步进行了皮尔逊相关分析。结果表明，混凝反应时间(R)与比表面积、吸液率呈负相关，与硅藻孔径呈正相关。指示凝块形成率的α角与硅藻大小和孔径呈负相关。此外，MA还与BET值呈负相关。本研究丰富了我们对硅藻在止血领域的应用潜力的认识，有助于加深我们对硅藻止血机理的理解。
It is increasingly essential to develop excellent materials for rapid hemorrhage control. Our previous study showed that centric diatoms such as frustules were superior to QuikClot® in hemostasis, however, related studies in pennate diatoms are still scarce. The morphological and physicochemical properties of pennate diatoms are quite different from those of centric diatoms, meaning that significant differences may also be observed from their hemostatic effects. Thus, the hemostasis effects of four pennate diatom frustules (Cocconeiopsis orthoneoides, Navicula avium, Navicula sp., and Pleurosigma indicum) were investigated in this study. Herein, all diatom frustules demonstrated outstanding hemostasis performance. For example, the in vitro coagulation time of C. orthoneoides (100.33 ± 9.5 s) was 32.4% lower than that of QuikClot®. Meanwhile, the hemostatic times of C. orthoneoides in the rat tail amputation and femoral artery models were 82 s and 180 s, respectively, only around one-half and one-third of the QuikClot® values. Moreover, the blood loss amounts of C. orthoneoides in the rat tail amputation and femoral artery model were 73.4% and 61% less than that of QuikClot®. Besides that, diatom frustules also exhibited favorable biocompatibility (hemolysis ratio <5%, MEFs cell viabilities >80%, and no inflammation). To find out the key factors underlying the hemostatic effect of frustules, Pearson correlation analysis was further performed in this study. The results demonstrated that the coagulation reaction time (R) was negatively correlated with the specific surface area and liquid absorbability but positively with the diatom pore diameter. The angle α, indicating the clot formation rate, was negative to the diatom size and pore diameter. Additionally, MA also showed a negative correlation with the BET value. This study can enrich our knowledge about the application potential of diatoms in the field of bleeding control and is helpful in deepening our understanding about the hemostatic mechanism of frustules.