Proanthocyanidins Rapidly Stabilize the Demineralized Dentin Layer
Proanthocyanidins Rapidly Stabilize the Demineralized Dentin Layer
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DOI：
10.1177/0022034513492769
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发表时间：
2013-08-01
影响因子：
7.6
通讯作者：
Wang, Y.
中科院分区：
文献类型：
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作者：
Liu, Y.;Dusevich, V.;Wang, Y.
虽然原花青素(PA)可有效提高胶原蛋白对胶原溶解降解的抵抗力，但将PA直接掺入粘合剂体系中对其光固化是不利的。相反，使用 PA 作为底漆可以避免这个问题，但人们对 PA 在以临床相关方式应用时稳定胶原蛋白的功效知之甚少。本研究调查了经过数秒级 PA 处理的酸蚀牙本质胶原蛋白层消化前和消化后的形态。必须拒绝原假设，即 PA 处理组和未处理对照组之间没有差异，因为研究表明，未经处理的对照组无法在外源胶原酶消化 1 小时后存活，而 PA 处理的胶原蛋白可以维持至少 16 小时的消化，并且胶原蛋白结构没有明显变化。此外，在可比较的实验条件下，金标准交联剂戊二醛的稳定作用被发现在允许的 5、15 或 30 秒交联内几乎不存在。因此，PA 已被证明在稳定脱矿牙本质胶原蛋白以对抗临床相关环境中的酶挑战方面非常有效，这可能是由于它们与胶原蛋白分子相互作用的非共价性质。
While proanthocyanidins (PA) are effective in improving collagen's resistance to collagenolytic degradation, the direct incorporation of PA into an adhesive system is detrimental to the light-curing thereof. Conversely, the use of PA as a primer could circumvent this issue, but little is known about the efficacy of PA in stabilizing collagen when applied in a clinically relevant manner. This study investigated the pre- and post-digestion morphology of an acid-etched dentin collagen layer that underwent PA treatment for time periods on a scale of seconds. The null hypothesis, that there is no difference between the PA-treated and untreated control group, had to be rejected, since it was revealed that the untreated control could not survive 1 hr of exogenous collagenase digestion, while the PA-treated collagen could sustain at least 16 hrs of digestion with no perceptible changes in collagen structure. In addition, the stabilizing effect of the gold-standard cross-linker glutaraldehyde at comparable experimental conditions was found to be almost non-existent within the 5, 15, or 30 sec of cross-linking permitted. Therefore, PA have been proven to be extraordinarily efficient in stabilizing demineralized dentin collagen against enzymatic challenges in a clinically relevant setting, likely due to the non-covalent nature of their interaction with collagen molecules.